宫颈癌组织中CIP2A和HPV16-E7的表达及其临床意义分析

目的 探讨宫颈癌(Cervical cancer)、宫颈上皮内瘤变(Cervical intraepithelial,CIN)及正常宫颈组织中CIP2A、HPV16-E7的表达及其之间的相互关系。方法 应用免疫组化S-P法检测50例宫颈鳞状细胞癌、34例CINⅠ、40例CINⅡ、45例CINⅢ和12例正常宫颈组织中CIP2A、HPV16-E7蛋白的表达。结果 CIP2A在宫颈癌、CINⅢ阳性表达率分别为68%(34/50)、13.3%(6/45);而在CINⅡ、CINⅠ及正常宫颈组织中阳性表达率均为0%。CIP2A蛋白在宫颈癌中的表达明显高于宫颈上皮内瘤变及正常宫颈组织,且与组织学分级、临床分期及淋巴结转移有关,差异均有统计学意义(P＜0.05)。HPV16-E7蛋白在宫颈癌、CINⅢ、CINⅡ及CINⅠ组织中的阳性表达率分别为72%(36/50)、68.89%(31/45)、67.50%(27/40)及64.71%(22/34);而在正常宫颈组织中阳性表达率为16.67%(2/12)。HPV16-E7蛋白在宫颈癌及宫颈上皮内瘤变组织中的表达明显高于正常宫颈组织,差异均有统计学意义(P＜0.05),且与年龄有关,而与组织学分级、临床分期及淋巴结转移无关。CIP2A 与HPV16-E7蛋白的表达呈正相关(r=0.514,P＜0.05)。结论 CIP2A、HPV16-E7在宫颈鳞状细胞癌中的过表达,提示宫颈癌中CIP2A与HPV16可能相互协调,共同促进宫颈癌的发生发展。

The expression and clinic significance of CIP2A and HPV16-E7 protein in cervical cancer

Objective To explore the expression and correlation of CIP2A and HPV16-E7 protein in cervical cancer.Methods The expressions of CIP2A and HPV16-E7 in 50 cases of cervical cancer,34 cases of CINⅠ,40 cases of CINⅡ,45 cases of CINⅢ and 12 cases of normal tissues were assessed by immunohistochemical method.Results The rate of expressions of CIP2A protein in cancer tissues and CINⅢ were 68%(34/50)and 13.3%(6/45)respectively;The rate of expressions of CIP2A protein in CINⅡ、CINⅠ tissues and normal tissues were negative.The expressions of CIP2A protein in cancer tissues were stronger than in CIN tissues and normal tissues(P＜0.05)and closely correlated with histologic grade,TNM stage and lymph node metastasis(P＜0.05).The rates of expressions of HPV16-E7 protein in cancer tissues CINⅢ,CINⅡand CINⅠ were 72%(36/50),68.89%(31/45),67.50%(27/40)and 64.71%(22/34)respectively.The rate of expressions of HPV16-E7 protein in normal tissues was 16.67%(2/12);The expressions of HPV16-E7 protein in cancer tissues and in CIN tissues were stronger than in normal tissues(P＜0.05)and closely correlated with age,but were not correlated with histologic grade,TNM stage and lymph node metastasis(P＞0.05).The expression of CIP2A was positively associated with the expression of HPV16-E7 in cervical cancer(r=0.514,P＜0.05).Conclusion The expressions of CIP2A and HPV16-E7 are very strong in cervical cancer;CIP2A and HPV16 may play important roles in the carcinogenesis and the development of cervical cancer.