血清miR-27b、miR-223水平与骨肉瘤患者化疗效果的相关性

目的:观察骨肉瘤患者血清微小RNA-27b、miR-223水平与化疗效果的相关性，指导未来临床对骨肉瘤患者实施合理的化疗。方法:选择我院2016年01月至2020年01月收治的241例骨肉瘤患者作为研究对象，均于化疗前检测血清miR-27b及miR-223水平，实施化疗治疗，分析血清miR-27b及miR-223水平与化疗效果的相关性。结果:241例骨肉瘤患者行化疗治疗后，总缓解率为59.75%；化疗后未缓解组肿瘤直径≥5 cm占比高于缓解组，化疗前血清miR-27b相对表达量高于缓解组，miR-223相对表达量低于缓解组，差异有统计学意义（P＜0.05）；两组其他基线资料比较，差异无统计学意义（P＞0.05）；经二元Logistic回归分析后建立多元回归模型，结果显示，骨肉瘤患者肿瘤直径、化疗前血清miR-27b及miR-223表达水平是化疗后未缓解的影响因素（OR＞1，P＜0.05）；绘制受试者工作曲线（ROC）发现，化疗前血清miR-27b及miR-223水平预测化疗后未缓解的曲线下面积（area under curve，AUC）均＞0.80，有一定预测性能。结论:骨肉瘤患者血清miR-27b、miR-223水平与化疗效果存在密切相关，化疗前血清miR-27b过表达及miR-223表达下调均可能提示化疗效果不佳，可将二者作为骨肉瘤患者化疗效果的有效预测因子，并根据评估结果合理调整治疗方案，可能对提高化疗整体获益意义重大。

Correlation between serum miR-27b and miR-223 levels and the effect of chemotherapy in patients with osteosarcoma

Objective:To observe the correlation between serum microRNA-27b,miR-223 levels and chemotherapy effects in patients with osteosarcoma,to guide the future clinical implementation of rational chemotherapy for patients with osteosarcoma.Methods:241 patients with osteosarcoma admitted to our hospital from January 2016 to January 2020 were selected as the research subjects.The serum miR-27b and miR-223 levels were detected before chemotherapy,and were all performed chemotherapy.To analyze the correlation between serum miR-27b and miR-223 levels and chemotherapy effects.Results:After 241 patients with osteosarcoma treated with chemotherapy,the total remission rate was 59.75%.After chemotherapy,the proportion of tumor diameter ≥5 cm in the non-remission group was higher than that in the remission group.The relative expression of serum miR-27b before chemotherapy was higher than that of the remission group.The relative expression of miR-223 was lower than that of the remission group,and the difference was significant statistically(P<0.05).Compared other baseline data between the two groups,the difference was not significant statistically(P>0.05).After binary logistic regression analysis,a multiple regression model was established.The results showed that tumor diameter in patients with osteosarcoma and the expression levels of miR-27b and miR-223 in serum before chemotherapy were the influencing factors of non-remission after chemotherapy(OR>1,P<0.05).The receiver operating curve(ROC) was drawn and found that the serum miR-27b and miR-223 levels before chemotherapy predicted the area under the curve(AUC) after chemotherapy were both>0.80,which had certain predictive performance.Conclusion:The serum miR-27b and miR-223 levels in patients with osteosarcoma are closely related to the effect of chemotherapy,the overexpression of serum miR-27b and the down-regulation of miR-223 expression before chemotherapy may indicate poor chemotherapy effect.Both can be used as effective predictors of the chemotherapy effect of patients with osteosarcoma,and the treatment plan can be adjusted reasonably based on the evaluation results,which may be of great significance to improving the overall benefit of chemotherapy.