高功率脉冲微波辐照对大鼠血清激素水平影响的研究

目的：通过对高功率脉冲微波（HPPMW）辐照后Wistar大鼠血清中睾酮（Testo)、雌二醇（E2）、促甲状腺激素（TSH）、游离甲状腺素T4（FT4）、皮质醇（Cort)、醛固酮（Ald)及生长激素（HGH）的动态观察，以探讨HPPMW对内分泌系统的影响。用高功率脉冲微波源，以功率密度为3050W／cm^2脉冲微波（频率为5．4GHz，脉宽为26ns)辐照辐照组动物。于辐照后1h,6h,24h,7d,14d及28d分别采血，用放射免疫法在FT630放免疫定仪上进行统一测定。所有数据经SPSS8．0统计处理。结果：大鼠被辐照后，其血清E2在早期变化不明显，但在14d时明显升高（P＜0．01）；Testo辐照后1h和14d明显下降（P＜0．05）；TSH于辐照后逐渐升高，14d到28d时明显增高（P＜0．05）；FT4辐照后1h明显升高（P＜0．05）；TSH于辐照后逐渐升高，14d到28d时明显增高（P＜0．05）；FT4辐照后1h明显升高（P＜0．05）后逐渐降低，在28d下降到最低点（P＜0．01）；Cort辐照后1h即升高，14d达到峰值（P＜0．05）；Ald于照后6h-14d略低于对照组，28d恢复；HGH辐照后明显低于照前水平，在28d下降到最低点（P＜0．05）。结论LHPPMW可引起大鼠血清激素水平的紊乱。既有早期影响，也表现为一定的持续效应。提示HPPMW可能直接引起大鼠内分泌系统的损伤。

Serum hormone levels in rats irradiated by high power pulse microwave(HPPMW)

ObjectiveWith observing the changes in testost er one (Testo),estradiol (E~{*-~}2), thyroidstimulating hormone (TSH),free thyronine (FT~{*-~}4), cortisol (Cort), aldosterone (Ald) and human growth hormone (HGH) leve ls in serum of rats irradiated by high power pulse microwave (HPPMW) to explore its influence on endocrine system. MethodsThirty_five male Wistar rats were randomly divided into seven groups,a normal control group and six HPPMW goups. HPPMW groups were i rradiated by 2 pulses (pulse duration was 26?ns)of 3?050?W/cm2 HPPMW with 5 minutes interval,while no treatmnt was given to control group.The left ventricl e blood was obtained at 1?h,~{*)~}6?h~{**~}, 24 ?h, 7?d, ~{*)~}14?d~{**~} and 28?d following irradiation, and were mea sured by FT630 automatic ~{*)~}radioimmunity~{**~} analyzer. All data were analyzed by statistical software SPSS8.0.ResultsThe CM(422 in seru m was increased significantly(P~}<0.01) on day 14,but unchanged at t he other stages.Testo was~CM)~ decreased significantly at 1?h and on day 14(P <0.05).TSH increased gradually following irradiation and increased significantly on day 14 and day 28 (P~{!<~}WT~{!=~}<0.05).FT~{*-~}4 decreased gradually and reached the lowest point on day 28(P~{!<~}WT~{!=~}<0.01) after increase at 1 ?h.The Cort was increased at 1?h and increased significantly on day 14(~{!<~}W TBX~{!=~}P~{!<~}WT~{!=~}<0.05).Ald had no different change compared with control (P ~{!<~}WT~{!=~}>0.05). HGH decreased gradually and reached the lowest point on day 28(P<0.05);ConclusionsHPPMW disturbance of serum hormones in rats and this effect appeared in early stage,and lasted a c ertain duration.It is inferred that HPPMW could injure the endocrine system of rats.