妊娠早期小鼠卵巢、输卵管及子宫内诱导型一氧化氮合酶的分布

目的　了解胚泡着床前后妊娠昆明系小鼠卵巢、输卵管及子宫内诱导型一氧化氮合酶 (inducible ni-tric oxide synthase,i NOS)的分布。　方法　免疫细胞化学 L SAB法。　结果　妊娠 2～ 5 d小鼠的卵巢内 ,黄体细胞上有 i NOS的阳性表达 ;输卵管粘膜上有 i NOS的分布 ,肌层则为阴性 ;妊娠 2、3d的小鼠子宫内 ,阳性标记主要出现在子宫内膜上皮以及子宫内膜中的子宫腺上皮 ,内膜基质细胞为阴性 ;妊娠 4d的子宫内 ,子宫腺及蜕膜部分均有 i-NOS的分布 ,妊娠 5 d时 ,在小鼠胚泡的表面也检测到了 i NOS的存在。　结论　小鼠胚泡着床前后 ,在其卵巢、输卵管及子宫内均有 i NOS的存在 ,提示 i NOS在小鼠胚胎早期发育及着床过程中起作用。

DISTRIBUTION OF iNOS IN THE OVARY,OVIDUCT AND UTERUS OF EARLY PREGNANT MICE

Objective To understand the distribution of iNOS in the ovary, oviduct and uterus of mouse(2 5 days of pregnancy). Methods Immunohistochemistry(LSAB) was used in this study. Results iNOS occured in the luteal cells of all detected ovaries. In the oviduct tissues, iNOS was only found in the mucosa. It also appeared in the epithelia of endometrium and the metrial glands of all detected uterus. In the decidua of both day 4 and day 5 pregnant mice and the day 5 embryo surface, there are the presence of iNOS. Conclusion iNOS can be found in the ovaries,oviducts and uterus during 2 5days of pregnant mice, which suggests that iNOS plays a role in the development of early mouse embryos and the implantation.