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| 反相高效液相色谱法测定复方五仁醇胶囊中2组分的含量 | |
| 引用本文： | 窦志华,丁安伟,施忠,李伟红,吴陈军,包贝华.反相高效液相色谱法测定复方五仁醇胶囊中2组分的含量[J].中国药房,2007,18(3):198-199. |
| 作者姓名： | 窦志华 丁安伟 施忠 李伟红 吴陈军 包贝华 |
| 作者单位： | 1. 南京中医药大学,南京市,210029 2. 南通市第三人民医院,南通市,226006 3. 南通市药品检验所,南通市,226006 |
| 基金项目： | 山西省体育局资助项目;江苏省南通市社会发展科技计划 |
| 摘 要： | 目的建立以反相高效液相色谱法测定复方五仁醇胶囊中五味子醇甲和五味子乙素含量的方法。方法色谱柱为Lichro-sphereC18,流动相为水-甲醇(梯度洗脱),流速为1.0mL.min-1,柱温为30℃,检测波长为254nm。结果五味子醇甲、五味子乙素进样量分别在0.1824~1.6416μg(r=1.0003)、0.1896~1.7064μg(r=0.9999)范围内与峰面积积分值线性关系良好;平均加样回收率分别为98.32%(RSD=1.02%)、97.22%(RSD=0.94%)。结论本方法简便、准确、专属性强,可用于本品的质量控制。 |
| 关 键 词： | 反相高效液相色谱法 复方五仁醇胶囊 五味子醇甲 五味子乙素 含量测定 |
| 文章编号： | 1001-0408（2007）03-0198-02 |
| 收稿时间： | 2006-04-01 |
| 修稿时间： | 2006-08-13 |
Determination of Schisandrin and Schisandrin B in Compound Wurenchun Capsule by RP- HPLC |
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| DOU Zhihua,DING Anwei,BAO Beihua,SHI Zhong,LI Weihong,WU Chenjun.Determination of Schisandrin and Schisandrin B in Compound Wurenchun Capsule by RP- HPLC[J].China Pharmacy,2007,18(3):198-199. | |
| Authors: | DOU Zhihua DING Anwei BAO Beihua SHI Zhong LI Weihong WU Chenjun |
| Abstract: | OBJECTIVE:To establish a method for content determination of schisandrin and schisandrin B in compound wurenchun capsule by RP-HPLC.METHODS:Column lichrosphere C18 was used,mobile phase of water and MeOH(gradient elution)was set up,the flow rate was 1.0mL·min-1,the column temperature was 30℃and the detection wavelength was 254nm.RESULTS:The linear ranges of schizandrin and schisandrin B were within 0.182 4～1.641 6μg(r=1.000 3)and 0.189 6～ 1.706 4μg(r=0 .999 9),respectively.The average recoveries were 98.32%(RSD=1.02%)and 97.22 %(RSD=0.94%),respectively.CONCLUSION:The established method is convenient,accurate and highly specific,and it can be used for the quality control of compound wurenchun capsule. |
| Keywords: | RP-HPLC Compound wurenchun capsule Schisandrin Schisandrin B Assaying |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
