泛素结合酶基因UbcH10在脑星形胶质细胞瘤组织中的表达及意义

目的 探讨泛素结合酶基因UbcH10在脑星形胶质细胞瘤组织中mRNA和蛋白表达水平及其与星形胶质细胞瘤病理级别的相关性. 方法 应用实时荧光定量PCR、免疫组织化学染色和蛋白印迹实验检测UbcH10 mRNA和蛋白在32例不同级别脑星形胶质细胞瘤和6例正常脑组织中的表达,并检测UbcH 10蛋白表达与增殖抗原Ki-67蛋白表达的相关性. 结果实时荧光定量PCR结果、免疫组织化学染色结果和蛋白印迹实验结果提示:与正常脑组织(1.00±1.57;O%;0.43±0.42)比较.UbcH10 mRNA和蛋白表达水平在高级别星形胶质细胞瘤(64.33±60.98;9.65%±5.75%;0.69±0.38)和低级别星形胶质细胞瘤(8.36±8.15;4.82%±3.30%;0.10±0.08)中明显上调,差异均有统计学意义(P<0.05);且高、低级别星形胶质细胞瘤之间比较差异也有统计学意义(P<0.05).UbcH10阳性细胞表达率与增殖抗原Ki-67阳性细胞表达率呈显著的正相关关系(r=0.67,P=0.000). 结论 泛素结合酶基因UbcH10可能在星形胶质细胞瘤的发生发展恶变过程中具有重要作用.

Expression of ubiquitin conjugating enzyme UbcH10 in brain astrocytomas

Objective To investigate the expression ofubiquitin conjugating enzyme UbcH10 gene at mRNA and protein levels in brain astrocytomas and its correlation to the pathological grades of astrocytomas. Methods Quantitative real-time RT-PCR analysis, immunohistochemistry and Western blot were performed to detect the expression of UbcH10 mRNA and protein in 32 astrocytomas of different pathological grades and 6 normal brain tissues. The correlation between UbcH10 and Ki-67 immunoreactivity was examined with the Spearman's correlation coefficient. Results Statistical analysis showed significantly increased expression levels of UbcH1O mRNA in high-grade astrocytornas (64.33±60.98) in comparison with those in low-grade astrocytomas (8.36±8.15) and normal brain tissues (1.00±1.57) (P<0.05). increased UbcH10 labeling index in immunohistochemistry was also noted in high-grade astrocytomas (9.65±5.75)%] as compared with that in low-grade astrocytomas (4.82± 3.30)%] (P<0.05) and normal brain tissues 0%, P<0.05). Western blot demonstrated significantly higher UbcH10 protein levels in high-grade astrocytomas (0.69±0.38) than in low-grade astrocytomas (0.10±0.08, P<0.05) and normal brain tissues (0.01±0.02, P<0.05). UbcH10 positivity rate was found to positively correlate to that of Ki-67 (r=0.67, P=0.000). Conclusion Overexpression of UbcH10 may play an important role in the development and progression of astrocytomas.