| Association of Chromosomal Telomere DNA With Nuclear Matrix in HeLa Cell |
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| 引用本文： | 汪国顺,罗文捷,潘惟钧,丁明孝,翟中和. Association of Chromosomal Telomere DNA With Nuclear Matrix in HeLa Cell[J]. 中国科学B辑(英文版), 1994, 0(6) |
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| 作者姓名： | 汪国顺 罗文捷 潘惟钧 丁明孝 翟中和 |
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| 作者单位： | Department of Biology，Peking University，Beijing 100871，PRC，Department of Biology，Peking University，Beijing 100871，PRC，Department of Biology，Peking University，Beijing 100871，PRC，Department of Biology，Peking University，Beijing 100871，PRC，Department of Biology，Peking University，Beijing 100871，PRC |
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| 摘 要： | Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly. Furthermore we found that chromosomal telomere DNA could bind to nuclear matrix specifically by the dot and Southern hybridization. The result of the Southwestern blot suggests that telomere DNA has high affinity to lamin B, vimentin and some nuclear matrix proteins. Therefore, the nuclear matrix and lamina of HeLa cell are possibly associated with spatial organization and action of chromosome.
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Association of Chromosomal Telomere DNA With Nuclear Matrix in HeLa Cell |
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| WANG Guo-Shun,LUO Wen-Jie PAN Wei-JunDING Ming-Xiao and ZHAI Zhong-He. Association of Chromosomal Telomere DNA With Nuclear Matrix in HeLa Cell[J]. Science in China(Chemistry), 1994, 0(6) |
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| Authors: | WANG Guo-Shun LUO Wen-Jie PAN Wei-JunDING Ming-Xiao ZHAI Zhong-He |
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| Abstract: | Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly. Furthermore we found that chromosomal telomere DNA could bind to nuclear matrix specifically by the dot and Southern hybridization. The result of the Southwestern blot suggests that telomere DNA has high affinity to lamin B, vimentin and some nuclear matrix proteins. Therefore, the nuclear matrix and lamina of HeLa cell are possibly associated with spatial organization and action of chromosome. |
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| Keywords: | chromosomal telomere nuclear matrix lamina Southwestern blot ESI (Electron Spectro-scopic Imaging). |
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