Mouririelliptica: Validation of gastroprotective,healing and anti-Helicobacter pylori effects |
| |
| Authors: | Fá bio Cruz Moleiro,Má rcio Aparecido Andreo,Raquel de Cá ssia dos Santos,Thiago de Mello Moraes,Clenilson Martins Rodrigues,Camila Bernardes de Andrade Carli,Flá via Cristine Mascia Lopes,Clá udia Helena Pellizzon,Iracilda Zeppone Carlos,Tais Maria Bauab,Wagner Vilegas,Clé lia Akiko Hiruma-Lima |
| |
| Affiliation: | 1. São Paulo State University - Departamento de Fisiologia, Instituto de Biociências, cp 610, CEP 18618-000, UNESP, Botucatu, SP, Brazil;2. São Paulo State University - Departamento de Química Orgânica, Instituto de Química, cp 355, CEP 14801-970, UNESP, Araraquara, SP, Brazil;3. São Paulo State University - Departamento de Análises Clinicas, cp 502, CEP 14801-902, UNESP, Araraquara, SP, Brazil;4. São Paulo State University - Departamento de Morfologia, Instituto de Biociências, cp 610, CEP 18618-000, UNESP, Botucatu, SP, Brazil;5. São Paulo State University-Departamento de Ciências Biológicas, cp 970, CEP 14801-970, UNESP, Araraquara, SP, Brazil |
| |
| Abstract: | Ethnopharmacological relevanceMouriri elliptica Martius (Melastomataceae) is species reputed in folk medicine to heal gastric ulcer and gastritis.Aim of the studyMethanolic extract (ME) and ethyl acetate fraction (EAF) from leaves of Mouriri elliptica were evaluated for their gastroprotective, healing, immunological, toxicological and anti-Helicobacter pylori activities.Material and methodsThe gastroprotective action of ME and EAF was evaluated in rodent experimental models and to elucidate mechanisms of action, the antisecretory action, involvements of NO, SH, PGE2, anti-Helicobacter pylori action of ME was evaluated. We also used immunohistochemical (PCNA and COX-2) and immunomodulatory (murine peritoneal macrophages) assays to evaluate Mouriri elliptica effects.ResultsME present gastroprotective action without antisecretory effect. Otherwise, ME showed anti-Helicobacter pylori action (MIC = 0.025 μg/mL) and was able to inhibit NO production by macrophages. This species also accelerate the healing of ulcerated gastric mucosa by stimulating proliferation factors (PCNA), COX-2 and maintained basal PGE2 level independent action of NSAID in gastric mucosa. The phytochemical investigation showed that this species possesses phenolic acid derivatives, acylglycoflavonoids and condensed tannins which probably influenced their pharmacological action.ConclusionAll these results suggest the efficacy and safety of Mouriri elliptica in combating and healing gastric ulcer. |
| |
| Keywords: | Mouriri elliptica Melastomataceae Antiulcer PCNA COX-2 Prostaglandin E2 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
