HPLC法同时测定大卫颗粒中绿原酸、连翘苷、黄芩苷的含量

目的:建立同时测定大卫颗粒中绿原酸、连翘苷、黄芩苷的高效液相色谱法。方法:采用Sinochrom ODS-BP（250mm×4.6mm,5μm）色谱柱,流动相为甲醇:30mmol·L^-1磷酸二氢钾溶液,梯度洗脱,流速为1.0ml·min^-1,检测波长为277nm。结果:绿原酸在10～80μg·ml^-1范围内线性关系良好（r=0.9996）,平均回收率为98.85%,RSD=1.02%（n=9）;连翘苷在10～80μg·ml^-1范围内线性关系良好（r=0.9993）,平均回收率为99.17%,RSD=0.99%（n=9）;黄芩苷50～600μg·ml^-1范围内线性关系良好（r=0.9995）,平均回收率为98.72%,RSD=1.10%（n=9）。结论:本方法简便易行,能够准确、快速测定大卫颗粒中的绿原酸、连翘苷和黄芩苷的含量。

Simultaneous Determination of Chlorogenic Acid, Phillyrin and Baicalin in Dawei Granules by HPLC

To establish an HPLC method for determining chlorogenic acid, phillyrin and baicalin in Dawei granules. Method： The separation column of Sinochrom ODS-BP （250 mm ×4. 6 ram,5 μm） was used,methanol-30 mmol·L^-1,K2HPO4 was applied as the mobile phase with gradient elution, the column temperature was 40 ℃, the detection wavelength was 277 nm and the flow rate was 1.0 ml·min ^-1. Result： The linear range of chlorogenic acid was 10-80μg. ml^-1 （ r = 0. 999 6） with the average recovery of 98.85% （ RSD = 1.02%, n = 9 ）, that of phillyrin was 10-80 μg·ml ^-1（ r = 0. 999 3 ） with the average recovery of 99. 17% （ RSD = 0. 99% n = 9 ）, and that of baicalin was 50-600μg. ml^-1 （ r = 0. 999 5 ） with the average recovery of 98. 72% （ RSD = 1.10% n = 9 ）. Conclusion： The method is simple, rapid and sensitive, which is suitable for the content determination of chlorogenic acid,phillyrin and baicalin in Dawei granules.