| 隐孢子虫病两种诊断方法的应用比较 |
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| 引用本文： | 徐梅倩,黄侠,朱顺海,黄燕,曹杰,何国声. 隐孢子虫病两种诊断方法的应用比较[J]. 中国兽医寄生虫病, 2007, 15(4): F0003-F0004 |
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| 作者姓名： | 徐梅倩 黄侠 朱顺海 黄燕 曹杰 何国声 |
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| 作者单位： | 中国农业科学院上海兽医研究所,上海,200232 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 目的对目前常用的两种检测隐孢子虫感染方法进行估价。方法取上海某牧场随机采集的20头奶牛粪便用改良抗酸染色法和巢式PCR(Nested PCR)法检测。结果两种方法均能检测出粪便中的隐孢子虫卵囊,改良抗酸染色法阳性检测率55%,Nested PCR法阳性检测率70%。结论:两种方法均能从感染隐孢子虫的奶牛粪便中捡出隐孢子虫卵囊,可根据需要选择。
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| 关 键 词： | 隐孢子虫 改良抗酸染色法 巢式PCR法 |
| 文章编号： | 1005-0868（2007）04-00封2-03 |
| 收稿时间： | 2006-09-14 |
| 修稿时间： | 2006-09-14 |
DETECTION OF CRYPTESPORIDIUM INFECTION BY TWO METHODS |
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| XU Mei-qian,XUANG Xia,ZHU Shun-hai,HUANG Yan,CAO Jie,HE Guo-sheng. DETECTION OF CRYPTESPORIDIUM INFECTION BY TWO METHODS[J]. Chinese Journal of Veterinary Parasitology, 2007, 15(4): F0003-F0004 |
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| Authors: | XU Mei-qian XUANG Xia ZHU Shun-hai HUANG Yan CAO Jie HE Guo-sheng |
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| Affiliation: | Shanghai Veterinary Research Institute, Shanghai 200232, China |
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| Abstract: | Objective To assess two methods for detection of Cryptesporidium infection.Methods Cryptesporidium in the feces of 20 milch cows from a cattle farm in Shanghai were detected by modified acid-fast staining and nested PCR.Results Two methods could detect Cyrptesporidium oocysts from random samples,and the positive rates of Cryptesporidium detected by modified acid-fase staining and nested PCR were 55% and 70% respectively.Condusion Two methods could detect Cryptesporidium oocysts from the feces of milch cows infected Cryptesporidium,and could be chosen by different methods. |
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| Keywords: | Cryptesporidium modified acid-fast staining nested PCR |
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