Cell pellets from dental papillae can reexhibit dental morphogenesis and dentinogenesis |
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Authors: | Yu Jin-Hua Shi Jun-Nan Deng Zhi-Hong Zhuang Heng Nie Xin Wang Ruo-Ning Jin Yan |
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Affiliation: | Department of Endodontics, College of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China. |
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Abstract: | We isolated dental papilla mesenchymal cells (DPMCs) from different rat incisor germs at the late bell stage and incubated them as cell pellets in polypropylene tubes. In vitro pellet culture of DPMCs presented several crucial characteristics of odontoblasts, as indicated by accelerated mineralization, positive immunostaining for dentin sialophosphoprotein and dentin matrix protein 1, and expression of dentin sialophosphoprotein mRNA. The allotransplantation of these pellets into renal capsules was also performed. Despite the absence of dental epithelial components, dissociated DPMCs with a complete loss of positional information rapidly underwent dentinogenesis and morphogenesis, and formed a cusp-like dentin-pulp complex containing distinctive odontoblasts, predentin, dentin, and dentinal tubules. These results imply that DPMCs at the late bell stage can reexhibit the dental morphogenesis and dentinogenesis by themselves, and epithelial-mesenchymal interactions at this stage may not be indispensable. Furthermore, different DPMC populations from the similar stage may keep the same developmental pattern. |
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Keywords: | Dental papilla Morphogenesis Dentinogenesis Epithelial-mesenchymal interaction Tissue engineering Positional information Pellet culture Odontoblast differentiation |
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