掺钕介孔硼硅酸盐生物活性玻璃陶瓷骨水泥的制备与性能表征

骨肉瘤是一种常见的恶性骨肿瘤, 常通过手术切除进行治疗。但术后造成的骨缺损难以自愈, 残余肿瘤细胞还会增加复发可能性。本研究开发了一种用于修复骨缺损和协同治疗骨肉瘤的掺钕介孔硼硅酸盐生物活性玻璃陶瓷骨水泥。首先通过溶胶-凝胶法结合固态反应制备了可作为光热剂和药物载体的掺钕介孔硼硅酸盐生物活性玻璃陶瓷微球(MBGC-xNd), 然后将微球与海藻酸钠(SA)溶液混合制备了可同时进行光热治疗和化学治疗的可注射骨水泥(MBGC-xNd/SA)。结果表明掺Nd³⁺赋予微球可控的光热性能, 负载阿霉素(DOX)的微球显示出持续的药物释放行为。此外, 载药骨水泥的药物释放量随着温度的升高而显著增加, 说明光热疗法产生的热量可促进DOX释放。体外细胞实验结果表明, MBGC-xNd/SA具有良好的促成骨活性, 并且光热-化学联合疗法对MG-63骨肉瘤细胞起到了更显著的杀伤作用, 表现出协同效应。因此，MBGC-xNd/SA作为一种新颖的多功能骨修复材料, 在骨肉瘤的术后治疗方面具有良好的应用前景。

Nd-doped Mesoporous Borosilicate Bioactive Glass-ceramic Bone Cement

As a common malignant bone tumor, osteosarcoma is usually treated by surgical resection. However, the bone defects caused by surgery are difficult to heal, and the possibility of osteosarcoma recurrence can also be increased by the residual tumor cells. Therefore, a Nd-doped mesoporous borosilicate bioactive glass-ceramic bone cement was developed for repair of bone defects and synergistic therapy of osteosarcoma. Firstly, as photothermal agent and drug carrier, Nd-doped mesoporous borosilicate bioactive glass-ceramic (MBGC-xNd) microspheres were prepared through Sol-Gel method and solid-state reaction. Then MBGC-xNd microspheres were mixed with sodium alginate (SA) solution to prepare injectable bone cement (MBGC-xNd/SA). The results showed that Nd³⁺ endows microspheres with controllable photothermal properties, and microspheres loaded with doxorubicin (DOX) showed sustained drug release behavior. In addition, the drug release from drug-loaded bone cement was significantly accelerated with the increase of temperature, indicating that the heat generated by photothermal therapy had the possibility of promoting the release of DOX. In vitro cell experiment results showed that MBGC-xNd/SA had good osteogenic activity. Simultaneously, photothermal-chemical combination therapy had a more significant killing effect on MG-63 osteosarcoma cells, indicating a synergistic effect. Therefore, MBGC-xNd/SA, as a novel multifunctional bone repair material, exhibits a potential application in the postoperative treatment of osteosarcoma.