| RP-HPLC法测定维生素E霜的含量 |
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| 引用本文： | 李坚,卢绮雯,何琳. RP-HPLC法测定维生素E霜的含量[J]. 郧阳医学院学报, 2006, 25(3): 155-157 |
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| 作者姓名： | 李坚 卢绮雯 何琳 |
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| 作者单位： | 广东药学院药科学院 广东广州510006 |
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| 摘 要： | 目的:测定维生素E霜中维生素E的含量。方法:采用反相高效液相色谱法,色谱柱:D iamonsilTM(钻石)C18柱,流动相:甲醇-氯仿(85∶15),流速:1.0 m l.m in-1,检测波长284 nm,柱温40℃。结果:维生素E的平均回收率为99.79%,方法精密度(RSD)为0.20%(n=6)。结论:该法可用于维生素E霜中维生素E的含量测定。
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| 关 键 词： | 维生素E霜 维生素E HPLC 含量测定 |
| 文章编号： | 1006-9674（2006）03-0155-03 |
| 收稿时间： | 2006-03-05 |
| 修稿时间： | 2006-03-05 |
Determination of Vitamin E in Vitamin E Cream by RP-HPLC |
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| LI Jian,LU Qi-wen,HE Lin. Determination of Vitamin E in Vitamin E Cream by RP-HPLC[J]. Journal of Yunyang Medical College, 2006, 25(3): 155-157 |
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| Authors: | LI Jian LU Qi-wen HE Lin |
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| Abstract: | ObjectiveTo determine the content of Vitamin E in Vitamin E Cream.Methods RP-HPLC method was used.The chromatographic column was Diamonsil~(TM) C_(18),the mobile phase was methanol∶chloroform(85∶15),the flow rate was 1.0ml·min~(-1),the detection wavelength was 284 nm,and the column temperature was 40 ℃.Results The average recoveries for Vitamin E was 99.79%,precision of the method was 0.20%(RSD,n=6).Conclusion The method could be used to determine the content of Vitamin E in Vitamin E Cream. |
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| Keywords: | HPLC |
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