| 内标校准液相色谱荧光法测定食用油中苯并（a）芘 |
| |
| 引用本文： | 王秀嫔,李培武,王琳,张文,张奇,甘冬生.内标校准液相色谱荧光法测定食用油中苯并（a）芘[J].中国油料,2011(6):603-608. |
| |
| 作者姓名： | 王秀嫔 李培武 王琳 张文 张奇 甘冬生 |
| |
| 作者单位： | [1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点开放实验室,湖北武汉430062 [3]农业部生物毒素检测重点实验室,湖北武汉430062 [4]农业部油料及制品质量监督检验测试中心,湖北武汉430062 |
| |
| 基金项目： | 国家油菜现代产业技术体系（cars-13）; 国家自然科学基金（31000877）; 农业部948项目（2010-G1 2011-G5）; 国家科技支撑计划（2010BAK08B03 2012BAB19B09）; 中国农业科学院油料作物研究所所长基金 |
| |
| 摘 要： | 采用内标苯并（b）屈校正,建立了正相固相净化-反相液相色谱-荧光分光光度法测定食用油中苯并（a）芘的方法。正相Plus Silica固相萃取柱作为净化柱,反相C18柱作为分离柱。以苯并（b）屈作为内标,测定了苯并（a）芘的校正因子。使用本方法,苯并（a）芘在液态食用油和固态食用油中的检出限分别为0.01和0.03μg/kg。苯并（a）芘在不同食用油回收率在83%～108%之间,方法的日内精密度和日间精密度分别小于7.7%和13.2%。
|
| 关 键 词： | 苯并（a）芘 苯并（b）屈 内标 正相固相净化 HPLC-FLD |
Determination of benzo[a]pyrene in edible oils by liquid chromatographyfluorescence detection with benzo[b]chrysene as internal standard |
| |
| Authors: | WANG Xiu-pin LI Pei-wu WANG Lin ZHANG Wen ZHANG Qi GAN Dong-sheng |
| |
| Affiliation: | 1.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China; 2.Key Laboratory of Oil Crop Biology and Genetic Improvement of the Ministry of Agriculture, Wuhan 430062,China;3.Key Laboratory of Biotoxins,MOA,Wuhan 430062,China; 4.Quality Inspection and Test Center for Oilseeds Products,MOA,Wuhan 430062,China) |
| |
| Abstract: | The normal-phase solid phase cleaning-reversed high performance liquid chromatography-fluorescence detection was applied for the determination of the benzoa]pyrene（BaP） in edible oils.The edible oil samples were cleansed with a Plus Silica solid phase extraction cartridge.The separation was carried out on a C18 column with gradient elution.With benzob]chrysene（BbC） as the internal standard,the relative correction factor（RCF） of BaP was determined by HPLC-FLD;the BaP was determined by using BbC as the single marker.The limits of detection were 0.01μg/kg and 0.03μg/kg for BaP in edible oils and in edible fats,respectively.Recoveries of BaP in different spiked edible oils were in the range from 83% to 108%.The intra-day precision and inter-day precision were less than 7.7% and 13.2% respectively. |
| |
| Keywords: | Benzo[a]pyrene Benzo[b]chrysene Internal standard Normal-phase solid phase cleaning HPLC-FLD |
| 本文献已被 维普 等数据库收录！ |
|
