内皮型一氧化氮合酶基因体内转染对大鼠颈总动脉损伤后血管狭窄程度的影响

目的 将内皮型一氧化氮合酶基因转染至球囊损伤后的大鼠颈总动脉中，观察其对血管狭窄程度的影响。方法 将大鼠分为正常对照组、空白对照组、pcDNA3．1（-）组和内皮型一氧化氮合酶组。除正常对照组外均行颈总动脉内膜球囊损伤术，术中采用脂质体栽体FuGENE6分别介导真核表达栽体pcDNA3．1-内皮型一氧化氮合酶和pcDNA3，1（-）转染至损伤后的内皮型一氧化氮合酶组和pcDNA3．1（-）组大鼠血管中。于术后2用处死大鼠，以逆转录-聚合酶链反应法检测转染后体外培养的血管平滑肌细胞中内皮型一氧化氮合酶mRNA的表达。并于术后1月和2月处死大鼠，检测颈总动脉血管狭窄的程度。结果 2周后转染pcDNA3．1-内皮型一氧化氮合酶基因的血管平滑肌细胞中可以检测出内皮型一氧化氮合酶mRNA的表达，1月和2月后转染pcDNA3．1-内皮型一氧化氮合酶基因的颈总动脉狭窄程度明显小于空白对照组和pcDNA3．1（-）组（P〈0．01）。结论 内皮型一氧化氮合酶基因体内转染可以在血管平滑肌细胞中成功表达，且可以明显改善球囊损伤后颈总动脉狭窄的程度。

The Influence of Endothelial Nitric Oxide Synthase Gene Transfection in Vivo on the Stenosis Degree of the Injured Rats' Common Carotid Arteries

Aim To transfect the endothelial nitric oxide synthase(eNOS) gene into the rats' common carotid arteries injured by catheter, then to observe the influence of the transfection on the stenosis degree of the vessles. Methods 55 male Wastar rats of 3 months age were divided into normal control group (n=10), blank control group (n=15), pcDNA3.1(-) transfection group (n=15) and eNOS trnsfection group (n=15). 45 rats were accepted the intimal catheter surgery of the left common carotid except the normal control group. After operation, pcDNA3.1(-) and pcDNA3.1-eNOS were transfected respectively into pcDNA3.1(-) and eNOS transfection groups by carrier of FuGENE 6. The rats were executed 2 weeks, 1 and 2 month after the operation, the transfected common carotid arteries were took out and stenosis degree of the arteries were measured. Results The eNOS mRNA could be detected in cultured smooth muscle cell and the stenosis degree was significantly decreased in eNOS transfection group (P<0.01). Conclusions eNOS gene could be transfected in vivo into the smooth muscle cell succesefully and the transfecton also could obviously decrease the stenosis degree of the catheter injuried arteries.