SDF-1对创缘表皮干细胞定向趋化及促创面愈合效应的研究

目的：观察干细胞趋化因子SDF-1在创面愈合过程中调控表皮干细胞定向迁移促进创面愈合的过程。方法：制作大鼠背部全层皮肤缺损模型,随机分为三个实验组：①SDF-1组;②AMD3100（CXCR4受体的拮抗剂）组;③空白对照组。分别于致伤后1天、3天、5天、7天和12天照相计算伤后不同时间创面占初始创面的百分比并取材。运用HE染色和免疫组化技术观察创缘表皮干细胞定向迁移分化的特点,寻找其规律。结果：与其他两个处理组相比,SDF-1处理组愈合时间缩短,其创缘皮表皮基底层细胞分裂增殖旺盛,创缘表皮干细胞数目明显多于其他两组。结论：在创面愈合过程中,SDF-1可以调控表皮干细胞向创缘迁移,加速创面上皮化。

SDF-1 accelerates wound healing via recruitment of epidermal stem cells

Objective To investigate the stem cell chemokine SDF-1 in the regulation of wound healing migration of epidermal stem cells to promote wound healing process.Methods Rat dorsal full-thickness skin defects were randomly divided into three experimental groups：①SDF-1 group;②AMD3100（CXCR4 receptor antagonist） group;③ control group.1 day,3 days,5 days,7 days and 12 days after injury camera calculates the wound at different times,and total percentage of the initial wound drawn.Using HE staining and immunohistochemical observation of a margin of epidermal migration and differentiation of stem cell characteristics,to find its own rules.Results Compared with the other two treatment groups,SDF-1 treatment to shorten healing time,the margin of skin epidermal basal cell division and proliferative margin of the number of epidermal stem cells was more than the other two groups.Conclusion In the wound healing process,SDF-1 can regulate epidermal stem cells migrate to the wound edge,accelerate wound epithelization.