| 长柱重楼中主要甾体皂苷类成分的定性定量分析 |
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| 引用本文： | 黄圆圆,康利平,彭华胜,刘大会,郝庆秀,赵加六,陈敏,黄璐琦. 长柱重楼中主要甾体皂苷类成分的定性定量分析[J]. 中国中药杂志, 2017, 42(18): 3452-3460 |
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| 作者姓名： | 黄圆圆 康利平 彭华胜 刘大会 郝庆秀 赵加六 陈敏 黄璐琦 |
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| 作者单位： | 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700;安徽中医药大学, 安徽 合肥 230012,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,安徽中医药大学, 安徽 合肥 230012,湖北中医药大学 药学院, 湖北 武汉 430065,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,兰坪县中盛生态产业有限公司, 云南 怒江 673100,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700,中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地, 北京 100700 |
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| 基金项目： | 国家"重大新药创制"科技重大专项（2014ZX09301306-001）;国家杰出青年科学基金项目（81325023）;中央级公益性科研院所基本科研业务费专项资金项目（ZZ0708117）;中央本级重大增减支项目（2060302） |
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| 摘 要： | 为了从化学成分上研究长柱重楼是否具有重楼药材代用品的开发价值,该研究利用UPLC-Q-TOF MS比较了长柱重楼和滇重楼的化学成分,并进一步利用HPLC-UV同时测定了77株不同来源的长柱重楼中8种重楼皂苷的含量。结果显示长柱重楼和滇重楼的化学成分相似,长柱重楼化学成分更为复杂,鉴定的22个主要共有峰均为甾体类化合物。不同株长柱重楼中重楼皂苷的含量具有一定的差异,其中药典规定4种甾体皂苷(重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ)总量最高的为3.30%,最低的为0.068%,8种甾体皂苷总量最高的为6.18%,最低的为0.71%。长柱重楼中78%的样品符合《中国药典》要求,整体质量较为稳定,可以作为重楼替代品种进行研究开发。
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| 关 键 词： | 重楼 长柱重楼 甾体皂苷 定性定量分析 |
| 收稿时间： | 2017-06-04 |
Qualitative and quantitative analyses of primary saponins in Paris forrestii |
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| HUANG Yuan-yuan,KANG Li-ping,PENG Hua-sheng,LIU Da-hui,HAO Qing-xiu,ZHAO Jia-liu,CHEN Min and HUANG Lu-qi. Qualitative and quantitative analyses of primary saponins in Paris forrestii[J]. China Journal of Chinese Materia Medica, 2017, 42(18): 3452-3460 |
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| Authors: | HUANG Yuan-yuan KANG Li-ping PENG Hua-sheng LIU Da-hui HAO Qing-xiu ZHAO Jia-liu CHEN Min HUANG Lu-qi |
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| Affiliation: | State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Anhui University of Chinese Medicine, Hefei 230012, China,State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Anhui University of Chinese Medicine, Hefei 230012, China,College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China,State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Lanping County in Ecological Industry Co., Ltd, Nujiang 673100, China,State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China and State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | In order to study whether Paris forrestii could be developed as a substitute of Paridis Rhizome, chemical compositions of P. forrestii and P. polyphylla var. yunnanensis were investigated by UPLC-Q-TOF MS. In addition, the contents of eight primary steroidal saponins in 77 batches of P. forrestii samples from different habitats were simultaneously determined by HPLC-UV. The results showed that P. forrestii and P. polyphylla var. yunnanensis have similar chemical compositions, and all 22 major common peaks were identified as steroid derivatives. Meanwhile, there were some differences in the contents of saponins in P. forrestii samples from different habitats. The contents of 4 steroidal saponins in Chinese Pharmacopoeia ranged from 0.068% to 3.30%, and the highest content of the 8 kinds of steroidal saponins was 6.18%, while the lowest was just 0.71%. Moreover, 78% of P. forrestii samples were in conformity with the requirements of Chinese Pharmacopoeia, indicating that P. forrestii samples had relatively stable quality and could be further studied as a substitute for Paridis Rhizome. |
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| Keywords: | Paris Paris forrestii steroidal saponins qualitative and quantitative analyses |
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