HPLC法同时测定氯甲醑中氯霉素和甲硝唑的含量

目的：建立HPLC法同时测定氯甲醑中氯霉素及甲硝唑的含量。方法：采用HPLC法,色谱柱为Nov-pak C18（150mm×4.6 mm,5μm）,流动相为甲醇∶水（75∶25）,流速为1.0 ml·min-1,检测波长为297 nm,柱温：30℃,进样量20μl。结果：甲硝唑和氯霉素线性范围分别为5~80μg·ml-1（r=0.999 7）和50~800μg·ml-1（r=0.999 8）;平均回收率分别为100.83%（RSD=1.82%,n=6）和100.20%（RSD=0.55%,n=6）。结论：本法快速方便、精密度高、重复性和稳定性好,可用于同时测定氯甲醑中氯霉素和甲硝唑的含量。

Simultaneous Determination of Chloromycetin and Metronidazole in Chlortalidone and Metronidazole Spirits by HPLC

Objective： To establish an HPLC method for the simultaneous determination of chloromycetin and metronidazole in chlortalidone and metronidazole spirits. Method： The HPLC method was performed on an NOV-pak C18（ 150 mm ×4.6 mm,5 μm）column and the mobile phase consisted of methanol and water（ 75∶ 25）. The flow rate was 1. 0 ml·ml- 1,the detection wavelength was 297 nm,the temperature of column was 30℃ and the sample size was 20 μl. Result： The calibration curve of metronidazole was linear within the range of 5-80μg·ml- 1（ r =0.999 7）,and the average recovery was100.83% with RSD of 1.82%（ n =6）. The calibration curve of chlortalidone was linear within the range of 50-800μg·ml- 1（ r =0.999 7）,and the average recovery was 100.2%with RSD of 0. 55%（ n = 6）. Conclusion： The method is simple,rapid,accurate and reproducible,and can be used in the quality control of chlortalidone and metronidazole spirits.