| 两种方法定位5个地点大豆蛋白质含量QTL |
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| 引用本文： | 单大鹏,刘春燕,蒋洪蔚,董晓慧,陈庆山,胡国华. 两种方法定位5个地点大豆蛋白质含量QTL[J]. 中国油料, 2011, 0(1): 9-14 |
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| 作者姓名： | 单大鹏 刘春燕 蒋洪蔚 董晓慧 陈庆山 胡国华 |
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| 作者单位： | [1]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [2]东北农业大学,黑龙江哈尔滨150030 [3]黑龙江省农科院绥化分院,黑龙江绥化152052 |
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| 基金项目： | 转基因专项大豆导入系构建及有利隐蔽基因挖掘（2009ZX08009-013B）; 农业部“引进国际先进农业科学技术”（2006G01）; 国家863计划（2006AA10Z1F4） |
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| 摘 要： | 利用CIM和MIM法,对大豆Charleston×东农594的154个重组自交系在5个不同地点进行蛋白质含量测定和QTL定位分析。共检测到25个QTL,分别位于A1、B1、C2、D1a、D1b、E、J、L和N连锁群上。用CIM法定位了20个与蛋白质含量相关的QTL,用MIM法定位了9个与蛋白质含量相关的QTL。在C2连锁群上多次定位的ProC2-4,标记区间为Sat_092-Satt460,与前人找到的QTL位点一致。
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| 关 键 词： | 大豆 蛋白质含量 QTL 多个环境 复合区间作图法 多重区间作图法 |
QTL analysis of soybean protein content using two methods in 5 different environments |
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| SHAN Da-peng,LIU Chun-yan,JIANG Hong-wei,DONG Xiao-hui,CHEN Qing-shan,HU Guo-hua. QTL analysis of soybean protein content using two methods in 5 different environments[J]. , 2011, 0(1): 9-14 |
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| Authors: | SHAN Da-peng LIU Chun-yan JIANG Hong-wei DONG Xiao-hui CHEN Qing-shan HU Guo-hua |
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| Affiliation: | 1.The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,China; 2.Northeast Agricultural University,Harbin 150030,China; 3.Suihua Branch of Heilongjiang Academy of Agricultural Sciences,Suihua 152052,China) |
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| Abstract: | Two mapping methods,composite interval mapping（CIM） and multiple interval mapping（MIM）,were employed to detect quantitative trait loci（QTL） of soybean protein content using 154 recombination inbred lines（RIL） population derived from a cross between Charleston and Dongnong 594 in five different locations.As a result,25 QTLs for protein content were mapped in linkage group A1,B1,C2,D1a,D1b,E,J,L,and N.In total,20 QTLs for protein content were detected by CIM,and 9 QTLs detected by MIM.Among the QTLs,ProC2-4 was mapped multiple times between Sat_092 and Satt460 on C2 linkage group,referred as a main effect QTL for soybean protein content. |
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| Keywords: | Soybean Protein content QTL Multiple sites CIM MIM |
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