Concentration and purification of galacto‐oligosaccharides using nanofiltration membranes |
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Authors: | Mariano Michelon Ana Paula Manera Andrea Limoeiro Carvalho Francisco Maugeri Filho |
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Affiliation: | 1. Laboratory of Bioprocess Engineering, Faculty of Food Engineering, University of Campinas (UNICAMP), , 13083‐862 Campinas, SP, Brazil;2. Food Microbiology and Toxicology Laboratory, Federal University of Pampa (UNIPAMPA), , 96413‐170 Bagé, RS, Brazil;3. Laboratory of Unit Operation, Technology Department, University of Feira de Santana (UEFS), , 44036‐900 Feira de Santana, BA, Brazil |
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Abstract: | This study was aimed at using nanofiltration to purify the galactooligosaccharides (GOS) found in a commercial mixture containing lactose, glucose and galactose, whose compositions were similar to that obtained through transgalactosylation reactions catalysed by β‐galactosidase from Kluyveromyces marxianus CCT 7082. NP030 polyethersulphone membrane was selected for the purification processes, once it differed statistically from other membranes due to the higher purification factor and permeate flux under the optimised temperature and pressure conditions of 35 °C and 3 MPa, respectively. Results indicate that NP030 membrane can be employed as a preliminary technique for the GOS purification with approximately 61% (w/w) of recovery. |
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Keywords: | Galacto‐oligosaccharides nanofiltration pressure and prebiotics |
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