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| Notch信号通路相关基因的表达与涎腺腺样囊性癌转移的关系 | |
| 引用本文： | 卢友光,佘林,林李嵩,张声,丁林灿.Notch信号通路相关基因的表达与涎腺腺样囊性癌转移的关系[J].福建医科大学学报,2009,43(2):89-93. |
| 作者姓名： | 卢友光 佘林 林李嵩 张声 丁林灿 |
| 作者单位： | 1. 福建医科大学,附属口腔医院,口腔预防科,福州,350002 2. 福建医科大学,附属第一医院,口腔科,福州,350005 3. 福建医科大学,附属第一医院,病理科,福州,350005 |
| 基金项目： | 福建省自然科学基金，福建省科技计划重点项目 |
| 摘 要： | 目的筛选与涎腺腺样囊性癌转移相关的Notch信号通路基因。方法对已构建的涎腺腺样囊性癌高低转移细胞株ACC-M、ACC-2基因表达谱进行生物信息学分析，在与Notch信号通路基因进行比对后，筛选出差异基因，通过荧光实时定量PCR技术对相关基因的表达差异进行初步验证。结果涎腺腺样囊性癌的表达谱中有46个基因与Notch信号通路有关。验证发现，Notch-1，Notch-2，Notch-4，CHUK，FOXC1，FZD2，FZD3，GBP2和RUNX1在2个细胞株中的表达差别有统计学意义（P〈0．05），同时Notch-1在发生转移的涎腺腺样囊性癌中的表达明显高于未发生转移的涎腺腺样囊性癌（P〈0．05）。结论Notch信号通路中的多个基因可能参与了涎腺腺样囊性癌的发生和发展；Notch-1的表达与涎腺腺样囊性癌的发生、转移密切相关。 |
| 关 键 词： | 癌 腺样囊性 涎腺肿瘤 聚合酶链反应 细胞 培养的 基因表达 信号传导 |
Gene Expression Profile of Notch Signaling Pathway and the Metastasis of Adenoid Cystic Carcinoma |
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| LU Youguang,SHE Lin,LIN Lisong,ZHANG Sheng,DING Lincan.Gene Expression Profile of Notch Signaling Pathway and the Metastasis of Adenoid Cystic Carcinoma[J].Journal of Fujian Medical University,2009,43(2):89-93. | |
| Authors: | LU Youguang SHE Lin LIN Lisong ZHANG Sheng DING Lincan |
| Affiliation: | LU Youguang1,SHE Lin1,LIN Lisong2,ZHANG Sheng3,DING Lincan1 1.Department of Preventive Dentistry,The Affiliated Stomatological Hospital,Fujian Medical University,Fuzhou 350002,China,2.Department of Stomatology,The Affiliated First Hospital,Fuzhou 350005,3.Department of Pathology |
| Abstract: | Objective To screen genes of Notch signaling pathway,which are related to the metastasis of adenoid cystic carcinoma. Methods The gene expression profile of adenoid cystic carcinoma cell lines with high and low metastasis potential,ACC-M and ACC-2,were conducted with bioinformatics analysis. By comparison with genes of Notch signaling pathway,differentially expressed genes were identified. Real time-PCR was used to examine the mRNA level of these genes in ACC-2 and ACC-M. Results 46 genes in the expression ... |
| Keywords: | carcinoma adenoid cystic salivary gland neoplasms polymerase chain reaction cells cultured gene expression signal transduction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
