雌激素对创伤失血性休克肠道细菌移位的影响

目的探讨雌激素对创伤失血性休克(Trauma/hemorrhageshock,T/HS)肠粘膜屏障损伤以及肠道细菌移位的影响。方法雌性青春期Wistar大鼠40只,随机分为卵巢切除组10只,对照组10只,雌二醇组10只,大剂量雌二醇组10只,观察T/HS60min,复苏4h后的肠粘膜损伤程度(Chiu氏评分),并取肠系膜淋巴结、肝组织及门静脉血进行细菌培养,改良鲎试剂法测定血浆内毒素含量。结果雌二醇组和大剂量雌二醇组与其他两组相比,肠粘膜Chiu氏评分显著降低,细菌培养计数及内毒素含量明显减少,P均<0.05。结论雌激素能改善创伤失血性休克大鼠的肠粘膜损伤程度,降低肠道细菌及内毒素移位的发生率。

Influence of Estrogen on Bacterial Translocation in Trauma-hemorrhage Shock

Objective To study the effect of estrogen on intestinal mucosal injury and bacterial translocation in trauma-hemorrhage shock. Methods Forty pubertal female rats were randomly divided into 4 groups namely ovariectomy group(n=10),control group(n=10),estradiol group(n=10)and high dose estradiol group (n=10). The rats were subjected to T/H S for 60 minutes,then reperfusion . Intestinal mucosal injury were depend on Chiu,s scores; mesenteric lymph nodes,liver,systemic blood were sent for culture;the plasma level of endotoxin was determined with limulus amebocyte lysate test four hours after resuscitation. Results There was significant difference in Chiu,s score between groups C ,D and two others. Significant difference also were found in bacterial translocation rate in mesenteric lymph node,liver systemic blood culture and in plasma endotoxin level.Conclusion Estrogen may protect the intestinal mucosal from injury in trauma-hemorrhage shock and reduce the probabilities of bacterial translocation .