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| BrdU标记在大鼠生精细胞体外培养中的应用 | |
| 引用本文： | 檀大羡,王植柔,刘锋,卢晟盛,李莲军,卢克焕,李佳荃.BrdU标记在大鼠生精细胞体外培养中的应用[J].中国现代医学杂志,2006,16(10):1508-1510,1513. |
| 作者姓名： | 檀大羡 王植柔 刘锋 卢晟盛 李莲军 卢克焕 李佳荃 |
| 作者单位： | 1. 广西壮族自治区计划生育研究服务中心,广西,南宁,530021 2. 广西医科大学附属肿瘤医院,泌尿外科,广西,南宁,530021 3. 南宁市第二人民医院,生殖医疗中心,广西,南宁,530031 4. 广西大学动物繁殖研究所,广西,南宁,530004 5. 广西医科大学医学实验中心,广西,南宁,530021 |
| 基金项目： | 广西科学研究与技术开发计划项目;广西南宁市科学研究与技术开发计划项目 |
| 摘 要： | 目的探讨应用5-溴-2-脱氧尿苷（5-bromo-2-deoxy-uridine，Brdu）标记检测大鼠生精细胞体外的分化、发育情况。方法采用BtdU体内标记生精细胞的DNA，免疫细胞化学法跟踪观察大鼠生精细胞在体外培养中的分化、发育情况。结果培养前在体内标记BrdU的细胞为初级精母细胞。经培养后在BrdU标记的细胞中出现早期精子细胞。结论大鼠生精细胞体外培养过程中，应用BrdU标记能检测生精细胞的分化、发育情况。 |
| 关 键 词： | 生精细胞 体外培养 |
| 文章编号： | 1005-8982（2006）10-1508-03 |
| 收稿时间： | 2005-10-20 |
| 修稿时间： | 2005-10-20 |
Application of BrdU-labeled in in vitro culturation of rat spermatogenic cells |
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| TAN Da-xian,WANG Zhi-rou,LIU Feng,LU Sheng-sheng,LI Lian-jun,LU Ke-huan,LI Jia-quan.Application of BrdU-labeled in in vitro culturation of rat spermatogenic cells[J].China Journal of Modern Medicine,2006,16(10):1508-1510,1513. | |
| Authors: | TAN Da-xian WANG Zhi-rou LIU Feng LU Sheng-sheng LI Lian-jun LU Ke-huan LI Jia-quan |
| Abstract: | Objective] To investigate differentiation and development of rat spermatogenic cells cultured in vitro by application of 5-bromo-2-Deoxyuridine(BrdU)-labeled immunocyto-chemistry.Methods] Rats were injected with BrdU 9 days before experiment in order to label the spermatogenic cells' DNA in vivo.The BrdU-labeled immunocytochemistry was used to observe spermatogenic cell differentiation and development in vitro.Results] BrdU-labeled immunocyto-chemistry showed that BrdU-labeled early phase spermatids were observed after the labeling primary spermatocytes cultured in vitro.Conclusion] BrdU-labeled immunocytochemistry can be used to detect the differentiation and development of spermatogenic cells culture in vitro. |
| Keywords: | BrdU |
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