血清中miR-155与乳腺癌的相关性 |
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引用本文: | 韩学东,张学营,甄林林,张建,任毅. 血清中miR-155与乳腺癌的相关性[J]. 中国肿瘤外科杂志, 2013, 5(1): 47-50 |
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作者姓名: | 韩学东 张学营 甄林林 张建 任毅 |
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作者单位: | 南京医科大学附属淮安第一医院普外科 |
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基金项目: | 淮安市科技局科研基金资助项目(No.HAS2011033) |
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摘 要: | 目的分析血清中miR-155的表达水平,探讨其与乳腺癌临床病理特征之间的关系。方法收集2010年12月至2011年4月入院的67例女性患者(乳腺癌45例,非乳腺癌22例)的血液标本,分离血清并提取miRNA,通过荧光实时定量PCR法检测血清中miR-155的表达水平,分析其与乳腺癌临床病理特征间的相关性。结果乳腺癌患者相比非乳腺癌患者,腋窝淋巴结转移阳性乳腺癌患者相比阴性患者血清中miR-155表达水平均上调(均P〈0.05);在乳腺癌患者中,肿瘤大小为T1的相比T2和r乃患者,I、Ⅱ期相比Ⅲ期患者,ER、PR阳性相比阴性患者血清中的miR-155表达水平均下调(均P〈0.05)。结论血清中miR.155的表达水平与乳腺癌及其临床病理特征密切相关。miR-155可成为新-代的乳腺癌标志物,并为乳腺癌发病机制及治疗和预后判断研究提供-个新方向。
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关 键 词: | 乳腺癌 miR-155 血清浓度 实时定量PCR 肿瘤标记物 |
Circulating miR-155 in serum of patients with breast cancer |
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Affiliation: | HAN Xuedong,ZHANG Xueying,ZHEN Linlin,et al. ( Department of General Surgery,Huai’an First People’s Hospital,Nanjing Medical University, Huai’an 223300,China) |
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Abstract: | Objective To investigate the expression of miR-155 in serum of patients with breast cancer and explore the possibility of miR-155 in serum as a marker of beast cancer. Methods 67 cases ( breast cancer 45 cases, non-breast cancer 22 cases) were enrolled from December 2010 to April 2011. The expression level of miR-155 in serum were measured by real-time quantitative polymerase chain reaction( real-time qPCR)analysis. The relationships between the expression level of miR-155 in serum and the feature of clinical pathology were an- alyzed. Results Compared with non-breast cancer, the expression level of miR-155 in serum of patients with breast cancer was up-regulated, The expression level of miR-155 was up-regulated in lymph positive breast cancer group compared with lymph negative breast cancer, The expression level of miR-155 was down-regulated in stage I and stage ]] breast cancer group compared with stage Ill breast cancer, The expression level of miR- 155 was down-regulated in serum of patients with ER and PR positive breast cancer compared with ER and PR negative breast cancer(P 〈 0.05). Conclusions This study demonstrates that the expression of miR-155 have a relationship with clinical pathological feature of patient with breast cancer and can be used as a potential tumor marker of breast cancer. |
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Keywords: | breast cancer miR-155 serum concentration real-time qPCR tumor markers |
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