| 维生素D3诱导的钙超载大鼠心肌肌浆网功能变化 |
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| 引用本文： | 齐永芬,董林旺,薛琳,王晓红,符明桂,唐朝枢.维生素D3诱导的钙超载大鼠心肌肌浆网功能变化[J].中国病理生理杂志,2001,17(4):289-292. |
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| 作者姓名： | 齐永芬 董林旺 薛琳 王晓红 符明桂 唐朝枢 |
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| 作者单位： | 北京医科大学第一医院心血管病研究所,北京 100034 |
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| 基金项目： | 国家自然科学基金资助(No.
39730220) |
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| 摘 要： | 目的:观察钙超载大鼠心肌肌浆网(SR)功能的变化。方法:腹腔注射维生素D3和nicotine灌胃复制大鼠心肌钙超载模型。差速离心制备心肌肌浆网,微孔过滤技术测定SR的钙释放与钙摄取,放射配基法测定SRryanodine受体结合能力。结果:钙超载大鼠心肌钙含量比对照组高324%(P＜0.01),心肌SR钙摄取和钙释放分别低64%和40%(P＜0.01)。心肌SRCa2+-ATP酶活性低65%(P＜0.01)。SR特异-ryanodine结合的最大值Bmax低51%(P＜0.01)。结论:钙超载大鼠心肌SR功能下降。
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| 关 键 词： | 钙 心肌 肌浆网 维生素D |
| 文章编号： | 1000-4718(2001)04-0289-04 |
| 收稿时间： | 1999-09-13 |
| 修稿时间： | 1999年9月13日 |
Alteration of cardiac sarcoplasmic reticulum function in vitamin D3-induced
calcium overload rats |
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| QI Yong-fen,DONG Lin-wang,XUE Lin,WANG Xiao-hong,FU Ming-gui,TANG Chao-shu.Alteration of cardiac sarcoplasmic reticulum function in vitamin D3-induced
calcium overload rats[J].Chinese Journal of Pathophysiology,2001,17(4):289-292. |
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| Authors: | QI Yong-fen DONG Lin-wang XUE Lin WANG Xiao-hong FU Ming-gui TANG Chao-shu |
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| Affiliation: | Institute of Cardiovascular Research, the First Hospital, Beijing Medical University, Beijing 100034, China |
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| Abstract: | AIM:To study alterations of cardiac sarcoplasmic reticulum (SR) function in vitamin D3-induced calcium overload rats. METHODS: The Ca-overload rat models were prepared by vitamin D3 plus nicotine. Cardiac SR was seperated by centrifuging. The measurement of SR Ca2+uptake and Ca2+ release activities were preformed by the Millimore filtration technique. Specific SR -ryanodine binding capacity was measured by radioligand method. RESULTS: Compared with control,myocardial calcium content in calcium overload rats increased by 78%(P＜0.01), SR Ca2+ uptake and Ca2+ release activities decreased by 64% and 40% respectively(P＜0.01),and in the meantime ,the Ca2+-ATPase activity decreased by 65%(P＜0.01).Maxmum value for -ryanodine binding decreased by 51%(P＜0.01). CONCLUSION:The function of cardiac SR in calcium-overload rats was decreased. |
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| Keywords: | Calcium Myocardium Sarcoplasmic reticulum Vitamin D |
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