|
|||||
|
|
Extremely high prevalence of multidrug resistant tuberculosis in Murmansk, Russia: a population-based study |
|
| Authors: | J M?kinen M Marjam?ki M Haanper?-Heikkinen H Marttila L B Endourova S E Presnova V Mathys P Bifani R Ruohonen M K Viljanen H Soini |
| Affiliation: | 1. Antimicrobial Resistance Unit, Mycobacterial Laboratory, National Institute for Health and Welfare, Kiinamyllynkatu 13, 20520, Turku, Finland 2. Murmansk Regional TB Dispensary, Murmansk, The Russian Federation 3. Molecular Pathology of Tuberculosis, Communicable and Infectious Diseases, Scientific Institute of Public Health, Brussels, Belgium 4. Finnish Lung Health Association, Helsinki, Finland 5. Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland |
| Abstract: | Drug resistance and molecular epidemiology of tuberculosis (TB) in the Murmansk region was investigated in a 2-year, population-based surveillance of the civilian population. During 2003 and 2004, isolates from all culture-positive cases were collected (n?=?1,226). Prevalence of multi-drug resistance (MDR) was extremely high, as 114 out of 439 new cases (26.0%), and 574 out of 787 previously treated cases (72.9%) were resistant to at least isoniazid (INH) and rifampin (RIF). Spoligotyping of the primary MDR-TB isolates revealed that most isolates grouped to the Beijing SIT1 genotype (n?=?91, 79.8%). Isolates of this genotype were further analyzed by IS6110 RFLP. Sequencing of gene targets associated with INH and RIF resistance further showed that the MDR-TB strains are highly homogeneous as 78% of the MDR, SIT1 strains had the same resistance-conferring mutations. The genetic homogeneity of the MDR-TB strains indicates that they are actively transmitted in Murmansk. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |
