| PKH-26标记骨髓间充质干细胞气管移植的体内示踪 |
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| 引用本文： | 韩云,庞超见,蓝妮,佟晓杰,石文君.PKH-26标记骨髓间充质干细胞气管移植的体内示踪[J].中国组织工程研究与临床康复,2011,15(45):8469-8473. |
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| 作者姓名： | 韩云 庞超见 蓝妮 佟晓杰 石文君 |
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| 作者单位： | 1. 中国医科大学附属盛京医院胸外科,辽宁省沈阳市,100004
2. 中国医科大学基础医学院解剖教研室,辽宁省沈阳市,110001 |
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| 基金项目： | 辽宁省教育厅科学技术研究项目(L2010606)资助~~ |
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| 摘 要： | 背景:PKH-26已经被成功应用于标记骨髓间充质干细胞并进行体内示踪。目的:通过气管移植合并静脉移植PKH-26标记的骨髓间充质干细胞评价PKH-26的示踪效果,并检测骨髓间充质干细胞在体内向受损组织的迁移情况。方法:用贴壁法进行骨髓间充质干细胞的原代细胞培养,以PKH-26标记3～5代骨髓间充质干细胞经鼠尾静脉移植入气管移植的受体大鼠体内,用等量的PBS注射作为实验对照。结果与结论:用PKH-26进行细胞骨髓间充质干细胞标记后,荧光显微镜下观察可见几乎所有骨髓间充质干细胞均有红色荧光。移植后8周仍可见受体气管和移植气管处有红色荧光标记。远离吻合口的受体气管基本未见红色荧光标记物。结果表明骨髓间充质干细胞经静脉移植后,可向损伤的气管组织迁移并定植,而且可随移植气管的血管化逐渐由受体气管切缘向移植气管迁移,最后可定植于移植段气管。PKH-26的红色荧光标记稳定性高,是可应用于长期观察的无降解的荧光标记物。
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| 关 键 词： | 气管移植 骨髓间充质干细胞 PKH-26 体内示踪 大鼠 |
Labeling and tracing bone marrow mesenchymal stem cells with PKH-26 in vivo |
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| Han Yun,Pang Chao-jian,Lan Ni,Tong Xiao-jie,Shi Wen-jun.Labeling and tracing bone marrow mesenchymal stem cells with PKH-26 in vivo[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(45):8469-8473. |
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| Authors: | Han Yun Pang Chao-jian Lan Ni Tong Xiao-jie Shi Wen-jun |
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| Affiliation: | Han Yun1,Pang Chao-jian2,Lan Ni2,Tong Xiao-jie2,Shi Wen-jun1 1Department of Thoracic Surgery,China Medical University Affiliated Shengjing Hospital,Shenyang 110004,Liaoning Province,China,2Department of Anatomy,School of Basic Medicine,China Medical University,Shenyang 110001 |
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| Abstract: | BACKGROUND:PKH-26 has been successfully applied to label bone marrow mesenchymal stem cells(BMSCs).OBJECTIVE:To evaluate the effect of PKH-26 on the labeling and tracing of BMSCs via tracheal transplantation and vein transplantation and to detect the migration of labeled BMSCs into the injured sites.METHODS:BMSCs were cultured by adherence method.PKH-26 labeled 3-5 passage BMSCs were injected into the recipient rats via the tail vein.Rats in the control groups were injected with the same amount of PBS.RESUL... |
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