| 有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响 |
| |
| 引用本文： | 李冬,何永平,张肖静,梁瑜海,张玉龙,范丹,张杰. 有机碳源对SNAD工艺脱氮性能及微生物种群结构的影响[J]. 哈尔滨工业大学学报, 2016, 48(2): 68-75 |
| |
| 作者姓名： | 李冬 何永平 张肖静 梁瑜海 张玉龙 范丹 张杰 |
| |
| 作者单位： | 水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京,水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京,郑州轻工业学院 环境污染治理与生态修复河南省协同创新中心,450001 郑州,水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京,水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京,水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京,水质科学与水环境恢复工程北京市重点实验室北京工业大学,100124 北京 ;城市水资源与水环境国家重点实验室哈尔滨工业大学,150090 哈尔滨 |
| |
| 基金项目： | 国家自然科学基金(51222807); 国家科技重大专项-水专项(2012ZX07202-005). |
| |
| 摘 要： | 为考察不同有机碳源质量浓度对亚硝化的全程自养脱氮工艺(SNAD)脱氮性能的影响,将该工艺应用到生活污水的处理中,采用MBR反应器,以葡萄糖作为有机物来源,通过逐步增大COD来实现,并运用PCR-DGGE技术研究了微生物种群结构的变化.反应器运行结果和DGGE图谱分析表明:碳氮比为0~2时,COD的增加不会抑制AOB和Anammox菌,AOB和Anammox菌的菌属种类不受影响,反而通过反硝化作用提高氮去除负荷.总氮去除率和氮去除负荷分别为67%和0.34 kg/(m3·d)左右.碳氮比为3~4及生活污水运行条件下,Anammox菌不受影响,AOB的活性受到抑制,菌属种类减少,脱氮效率下降.生活污水运行阶段,总氮去除率和氮去除负荷平均分别为73%和0.17 kg/(m3·d).Nitrosomonas和Candidatus Kuenenia stuttgartiensis一直是反应器内的优势菌属,共同完成脱氮过程.
|
| 关 键 词： | 同步亚硝化厌氧氨氧化和反硝化 有机物 生活污水 聚合酶链式反应-变性梯度凝胶电泳 微生物 |
| 收稿时间： | 2014-08-08 |
Effect of organic carbon on nitrogen removal and the microbial communities in SNAD process |
| |
| LI Dong,HE Yongping,ZHANG Xiaojing,LIANG Yuhai,ZHANG Yulong,FAN Dan and ZHANG Jie. Effect of organic carbon on nitrogen removal and the microbial communities in SNAD process[J]. Journal of Harbin Institute of Technology, 2016, 48(2): 68-75 |
| |
| Authors: | LI Dong HE Yongping ZHANG Xiaojing LIANG Yuhai ZHANG Yulong FAN Dan ZHANG Jie |
| |
| Affiliation: | Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China,Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, 450001 Zhengzhou,China,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China and Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery EngineeringBeijing University of Technology, 100124 Beijing,China ;State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of Technology, 150090 Harbin,China |
| |
| Abstract: | Effect of different organic substrate concentration on nitrogen removal in SNAD and the application of this process to treat domestic wastewater were investigated in a MBR, with gradually increased glucose as a source of organic matter. Besides,changes of microbial communities were observed by PCR-DGGE techniques. Experimental results and DGGE profiles showed that the increase of organic carbon concentration did not inhibited the activity and category species of AOB and Anammox bacteria when the C/N ration was ranged 0 to 2.0. Meanwhile, a significantly improvement of nitrogen removal was observed via effective denitrification, with a total nitrogen removal efficiency of 67% and nitrogen removal rate of 0.34 kg/(m3d), respectively. Under the C/N of 3-4 condition, the anammox bacteria in the system changed insignificantly,but AOB was inhibited and the category species reduced, resulting a decreasing of nitrogen removal efficiency.Total nitrogen removal efficiency and nitrogen removal rate were about 73% and 0.17 kg/(m3d), respectively.Nitrosomonas and Candidatus Kuenenia stuttgartiensiswere the predominant microorganisms in the SNAD reactor and played a major role in autotrophic nitrogen removal. |
| |
| Keywords: | SNAD organic carbon domestic wastewater PCR-DGGE microbes |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《哈尔滨工业大学学报》浏览原始摘要信息 |
|
点击此处可从《哈尔滨工业大学学报》下载全文 |
|
