| 高产β-甘露聚糖酶黑曲霉菌株的分离与鉴定 |
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| 引用本文： | 王佰涛,刘德海,陈晓飞,刁文涛,李珊珊,雷高.高产β-甘露聚糖酶黑曲霉菌株的分离与鉴定[J].中国酿造,2022,41(5):101-105. |
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| 作者姓名： | 王佰涛 刘德海 陈晓飞 刁文涛 李珊珊 雷高 |
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| 作者单位： | （1.河南省科学院生物研究所有限责任公司，河南 郑州 450008；2.河南省微生物工程重点实验室，河南 郑州 450008） |
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| 摘 要： | 该研究以采集的河南南阳魔芋种植土壤样品为试验材料，通过富集培养、测定产β-甘露聚糖酶酶活力，分离筛选高产β-甘露聚糖酶的菌株，并通过形态观察、内转录间隔区（ITS）基因和β-微管蛋白基因BenA序列分析对其进行菌种鉴定。结果表明，筛选得到一株高产β-甘露聚糖酶真菌菌株HKS016，其β-甘露聚糖酶酶活力为2.67 U/mL，该菌株被鉴定为黑曲霉（Aspergillus niger），保藏于中国普通微生物学菌种保藏中心（CGMCC），保藏编号为CGMCC No.22413。
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| 关 键 词： | β-甘露聚糖酶 分离 鉴定 黑曲霉 内转录间隔区 β-微管蛋白基因 |
Isolation and identification of high-yield β-mannanase Aspergillus niger |
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| WANG Baitao,LIU Dehai,CHEN Xiaofei,DIAO Wentao,LI Shanshan,LEI Gao.Isolation and identification of high-yield β-mannanase Aspergillus niger[J].China Brewing,2022,41(5):101-105. |
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| Authors: | WANG Baitao LIU Dehai CHEN Xiaofei DIAO Wentao LI Shanshan LEI Gao |
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| Affiliation: | (1.Institute of Biology Co., Ltd., Henan Academy of Science, Zhengzhou 450008, China; 2.Henan Key Laboratory of Microbial Engineering, Zhengzhou 450008, China) |
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| Abstract: | In this study, using soil samples collected from konjac cultivation in Nanyang, Henan province as test materials, the high-yield β-mannanase strains were isolated and screened by enrichment culture and determination of β-mannanase activity, and the strains were identified by morphological observation, internal transcribed spacer (ITS) gene and β-tubulin gene BenA sequence analysis. The results showed that a fungal strain HKS016 with high-yield β-mannanase was screened, and its β-mannanase activity was 2.67 U/ml. The strain was identified as Aspergillus niger and stored in the the China General Microbiological Culture Collection Center (CGMCC), numbered as CGMCC No.22413. |
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| Keywords: | β-mannanase isolation identification Aspergillus niger internal transcribed spacer β-tubulin gene |
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