丁基锡化合物与人血清白蛋白相互作用的研究

通过紫外、荧光和CD光谱, 研究了船体防污漆中主要防污成分——三丁基锡(TBT)化合物及其降解产物——一丁基锡(MBT)和二丁基锡(DBT)与人血清白蛋白(HSA)相互作用的方式与程度, 考察了浓度、酸度、有机溶剂和离子强度等因素对相互作用的影响. 结果表明, 丁基锡与HSA的相互作用是双重的, 既有丁基锡中锡离子与HSA的配位作用, 又有丁基锡中丁基基团的疏水作用, 导致HSA二级结构破坏, α-螺旋结构减少和构象转变. 一般情况下, MBT以配位作用为主, TBT以疏水作用为主, DBT两者兼而有之.

Study on Interaction of Butyltin Compounds withHuman Serum Albumin

The interaction of tributyltin (TBT), the main components of antifouling paint of ships, and its degradation products of monobutyltin (MBT) and dibutyltin (DBT) with human serum albumin (HSA) was investigated by ultraviolet, fluorescence and circle dichromism spectra. The influences of the concentration, acidity, organic solvent such as ethanol and ionic strength on the interaction of butyltin compounds and HSA molecule were examined to find that the interaction of butyltin compounds and HSA was dual. The butyltin compounds had not only coordinate but also hydrophobic actions with HSA molecule, which led to the destruction of the secondary structure of HSA molecule, decrease of content of α-spiral structure and transformation of conformation. Generally, MBT is inclined to act with HSA molecule through coordination action, while TBT is inclined to do with HSA molecule through hydrophobic action, and DBT can act with HSA molecule adopting both actions synchronously.