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| HPLC法测定羟甲烟胺的含量 | |
| 引用本文： | 武玉敏,李大伟,李云霞. HPLC法测定羟甲烟胺的含量[J]. 齐鲁药事, 2005, 24(1): 30-31 |
| 作者姓名： | 武玉敏 李大伟 李云霞 |
| 作者单位： | 1. 山东山大康诺制药有限公司,济南,250012 2. 山东省生物药物研究院,济南,250108 3. 济南市第二人民医院,济南,250001 |
| 摘 要： | 目的 采用高效液相色谱法测定羟甲烟胺的含量。方法 以C18化学键合硅胶柱分离羟甲烟胺,以甲醇 -磷酸二氢钠(pH =6.0 )(10∶90 )为流动相,UV检测波长 2 6 1nm进行测定。结果 平均回收率为 99 76 %,RSD为 0 2 9%(n =5),羟甲烟胺进样量与吸收峰面积呈良好的线性关系。线性范围 0 1～ 0 6mg·ml-1。结论 该方法测定羟甲烟胺的含量,结果准确,重现性好。 |
| 关 键 词： | HPLC 羟甲烟胺 含量测定 |
| 文章编号： | 1672-7738(2005)01-0030-02 |
Determination of Hydroxymethylnicotinamide with HPLC |
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| Wu Yu-min,Li Da-wei,Li Yun-xia. Determination of Hydroxymethylnicotinamide with HPLC[J]. qilu pharmaceutical affairs, 2005, 24(1): 30-31 | |
| Authors: | Wu Yu-min Li Da-wei Li Yun-xia |
| Affiliation: | Wu Yu-min,Li Da-wei1,Li Yun-xia2 |
| Abstract: | Objective The content of Hydroxymethylnicotinamide was determined by HPLC.Methods Hydroxymethylnicotinamide was separated by C 18-silica gel column with methonal-natrium phosphate dibasic solution(pH=6.0)(10∶90) as the eluent,UV determination wave-length was 261nm.Results Average recovery of the method was 99.76%,RSD=0.29%(n=5),the content of Hydroxymethylnicotinamide showed good linear relation with peak areas,linearity range was 0.1～0.6mg·ml -1. Conclusion This method was accurate and suitable for Hydroxymethylnicotinamide content determination. |
| Keywords: | HPLC Hydroxymethylnicotinamide determination. |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
