| PCR-SSP法检测人胰腺癌细胞株PCNA-1的K-ras基因点突变的方式 |
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| 引用本文： | 王伟,王春友,董继华,赵刚,陈雄,张敏.PCR-SSP法检测人胰腺癌细胞株PCNA-1的K-ras基因点突变的方式[J].中德临床肿瘤学杂志,2006,5(1):46-48. |
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| 作者姓名： | 王伟 王春友 董继华 赵刚 陈雄 张敏 |
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| 作者单位： | 武汉华中科技大学同济医学院附属协和医院胰腺外科中心 430022 |
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| 摘 要： | 目的检测人胰腺癌细胞株PANC-1的K-ras基因点突变方式,明确干扰Ras基因靶点的碱基序列。方法针对K-ras基因第十二位点密码子点突变最常见方式CAT,CGT,GTT设计顺序特异性引物(SSP),对人胰腺癌细胞株PANC1进行聚合酶链反应(PCR),扩增产物用聚丙烯酰胺凝胶电泳判定该细胞株有无点突变及突变方式。结果人胰腺癌细胞株PANC-1存在K-ras基因的点突变,其突变方式为CAT。结论 PCR-SSP法快速简便,特异性高,结果准确,为应用RNAi技术研究胰腺癌的基因治疗奠定了基础。
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| 关 键 词： | 聚合酶链反应 胰腺肿瘤 K-ras基因 点突变 |
| 收稿时间： | 5 January 2005 |
| 修稿时间： | 25 June 2005 |
Detection of K-ras Gene Point Mutation's Style in Human
Pancreatic Cancer Cell Line PANC-1 by PCR-SSP |
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| Wei WANG,Chunyou WANG,Jihua DONG,Gang ZHAO,Xiong CHEN,Min ZHANG.Detection of K-ras Gene Point Mutation's Style in Human
Pancreatic Cancer Cell Line PANC-1 by PCR-SSP[J].The Chinese-German Journal of Clinical Oncology,2006,5(1):46-48. |
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| Authors: | Wei WANG Chunyou WANG Jihua DONG Gang ZHAO Xiong CHEN Min ZHANG |
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| Affiliation: | (1) Department of Pancreas Surgery Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China;(2) Department of Central Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China;(3) Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China;(4) Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China |
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| Abstract: | To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods: Three kinds of special sequence primers (SSP) for polymerase chain reaction (PCR) with regard to the mutation styles (GAT, CGT and GGT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line PANC-1. The amplification products were studied with polyacrylamine gel electrophoresis to detect the style of point mutation. Results: The style of K-ras gene point mutation at codon 12 was GAT in human pancreatic cancer cell line. Conclusion: PCR-SSP is rapid, convenient and high specific. The results provide a basis for further gene therapy by RNA interference for pancreatic cancer. |
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| Keywords: | pancreatic cancer K-ras gene point mutation polymerase chain reaction |
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