小麦地方品种淀粉颗粒结合蛋白及淀粉含量差异研究

淀粉颗粒结合蛋白包含了多种淀粉生物合成的关键酶,对作物淀粉品质有重要影响。本研究利用1D-SDS-PAGE,分离了74份四川、西藏及云南毗邻地区小麦的淀粉颗粒结合蛋白,对突变材料进行了分子标记检测,对总淀粉和直链淀粉含量差异进行了比较。发现供试材料中,在分子量57～130 kDa区域共有9种不同的蛋白条带。其中,2个条带可能为新的淀粉颗粒结合蛋白;存在12份Wx-B1缺失的自然突变体和3份稀有的SGP-B1缺失突变体;筛选到直链淀粉含量超过30%的材料2份,直链淀粉含量为15%左右的材料10份。这些材料为小麦淀粉品质改良及淀粉生化合成机理研究提供了基础。

Studies on Starch Granule-Associated Proteins and Starch Content in Local Wheat

The majority of Starch granule-associated proteins (SGAPs) are believed to correspond to starch biological enzymases and identification and characterization of SGAPs are beneficial to the finding of novel mutants, which is very important to understand better the mechanisms of starch biosynthesis and improvement of starch quality in the breeding programms. In this study, we firstly Separated SGAPs of 74 indigenous wheat accessions from the adjacent areas of Sichuan, Tibet and Yunnan of China using 1D-SDS-PAGE technique and analysed starch and amylose content . A total of 9 different bands with molecular weights 57~130 kD, were found, of which bands 1and 2 were novel protein bands and 12 mutants with the deletion of Wx-B1 and 3 rare mutants with the deletion of SGP-B1(SSII ) were presented. The amylose content of the studied accessions was between 14.73% and 40.73%, with an average of 20.39%, and that of 10 materials was about 15% and 2 over 30%.These results are very important for further study on the of starch synthesis mechanism and improvement of starch quality.