Extracorporeal Endotoxin Removal by Immobilized Polyethylenimine

Abstract: The neutralization of bacterial endotoxins (ET) is still an unsolved problem in therapeutic medicine. The efficacy of antiendotoxin antibodies or receptor antagonists and other substances interferring with the endotoxininduced pathomechanisms is dependent on an intact cellular degradation system of the host. However, the phagocytosis function of that system seems to be impaired regularly in patients with intense or long-lasting endotoxemia or septic shock and in patients undergoing hemodialysis. Extracorporeal adsorption of ET might well be an effective support in the anti-ET therapy by lowering the amount of circulating ET and thus relieving the defense system of the body. In this work a new ET-adsorbent based on macroporous cellulosic beads with immobilized polyethylenimine (PEI) was tested for its ET-removal capacity in vitro. A test solution with 100 ng/ml ET from Escherichia coli 055:B5 was recirculated in a system containing the adsorbent beads. Polymyxin B immobilized to the same carrier was used for comparison. PEI as well as polymyxin B showed complete removal of ET from plasma and water as was measured by the Limulus Amebocyte Lysate (LAL) test (Chromogenix). The biocompatibility of the PEI adsorber was superior to that of polymyxin B. The results indicate that the PEI adsorber is of high efficacy and possibly of interest for the treatment of endotoxemia.