停服棉酚后睾丸活检及精液观察

用光镜检查服棉酚、不育及正常人(各2例)睾丸活检,结果:不育者睾丸曲细精管管腔闭锁,基膜增厚,透明样变,腔内以支持细胞为主;生精上皮细胞疏松变薄,组合紊乱,并中止于精子细胞阶段。服棉酚者睾丸萎缩,多数曲细精管退化消失,各级生精细胞消失,仅存支持细胞,且呈空网状结构。间质胶原纤维增生,间质细胞和小血管变性,大量淋巴细胞浸润。电镜观察2例服棉酚1和4年后,停药7和1年精液标本。发现精液中生精细胞和畸形精子仍多,但细胞质内多膜层结构,线粒体呈空泡状,顶体和尾部均不发育。

TESTICULAR BIOPSIES AND SEMEN EXAMINATIONS AFTER GOSSYPOL TREATMENT

6 testicular biopsies were studied under the light microscope. 2 had taken gossypol (one for 5 months, then stopped for 64 months; the other for 2 years, stopped for 2 years), 2 were infertile, and 2 were normal adults. After comparison with the normal testis results showed (1) After gossypol administration, there was testicular atrophy. Most seminiferous tubules had degenerated and vanished. In some, seminiferous epithelia disappeared, and only empty reticular sertoli cell structures remained. There was proliferation of interstitial collagen, degeneration of interstitial cells and small blood vessels, and lymphocytic infiltration; (2) In one of the infertile cases, seminiferous epithelial cells in the seminiferous tubules became loose, thin and disorderly. The basilemma in other seminiferous tubules became thicker and hyali-nized. Spermatogenesis was arrested in the spermatid stage. There was coalescence in some of the and seminiferous tubular cavities. With only some sertoli cells remaining. 2 semen samples from men who had taken gossypol for 0.5-5 years and stopped for 2-7 years were examined under the electronic microscope. Many spermatogenic cells and aberrant spermatozoo were seen. Multiple laminated membranous structures existed in the cytoplasm, and there was mitochondriac vacuolization with non-development of the acrosome and the tail.