人纤溶酶原kringle5功能区基因在大肠杆菌中克隆和表达

目的:构建含有人纤溶酶原kringle5功能区基因的表达质粒,了解在E.coli中表达情况。方法:采用基因工程技术将人纤溶酶原kringle5功能区基因插入载体PBV220E.coRI位点,再转化到E.coliTGI中,SDS-PAGE观察基因表达。结果:构建出表达质粒PBVK5,42℃诱导获得表达。结论:人纤溶酶原kringle5功能区基因表达产物以可溶性蛋白形式存在,表达量占菌体总蛋白9.8%。

Cloning and expression of human plasminogen kringle 5 gene in E.Coli

AIM: Constructing plasmid that expresses human plasminogen kringle 5 gene to analyze the gene expression in E.coli. METHODS: The gene of human plasminogen kringle 5 was inserted into plasmid PBV220 EcoRI site by gene manipulation techniques and was transformed to E.coli TGI. The gene expression was observed by SDS-PAGE. RESULTS: Expression vector PBVK5 was constructed, and human plasmingen kringle 5 gene product was obtained at 42℃ induction. CONCLUSION: Expression product of human plasminogen kringle 5 gene was soluble form of proteins, and the expression amount was 9.8% in E.coli TGI total proteins.