| 诱导多能干细胞体外向生精细胞自发分化潜能的研究 |
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| 引用本文： | 李朋,胡洪亮,张玲玲,丁慧,张伟,田汝辉,刘平,史占平,郭熙志,李铮,黄翼然.诱导多能干细胞体外向生精细胞自发分化潜能的研究[J].中华男科学杂志,2011,17(1):8-12. |
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| 作者姓名： | 李朋 胡洪亮 张玲玲 丁慧 张伟 田汝辉 刘平 史占平 郭熙志 李铮 黄翼然 |
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| 作者单位： | 1. 上海交通大学医学院附属仁济医院泌尿外科,上海市男科学研究所精子发育与遗传学实验室,上海市人类精子库,上海,200001
2. 上海交通大学Bio-X中心,上海,200240 |
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| 基金项目： | 国家重点基础研究发展计划(973计划,2010CB945200); 上海市教委重点课题(10ZZ70); 上海交通大学医学院自然基金课题(2008XJ017)~~ |
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| 摘 要： | 目的:探讨诱导多能干细胞(iPS)体外培养自发分化过程中生精细胞相关基因的表达,评估iPS体外向生精细胞自发分化的潜能。方法:经类胚体(EB)形成,体外诱导iPS向生精细胞分化,实时定量PCR和PCR检测生精细胞相关基因的表达。结果:实时定量PCR和PCR结果显示iPS经EB形成诱导分化后生精细胞不同时期的相关基因均有不同程度表达。iPS体外培养自发分化后生精细胞相关基因出现4种时间表达特征:Oct4基因表达量呈波浪状上升;Dppa3和Stra8基因表达量随诱导时间延长而下降;Dazl基因表达量呈波浪状下降;减数分裂前期基因Tex14、Msy2,减数分裂期基因Scp1、Scp3以及单倍体基因Akap3随着诱导时间延长先表达增加,而后表达下降。结论:iPS在经EB自发分化过程中表达生精细胞不同时期的相关基因,并且表达雄性配子单倍体基因,具有向雄性配子的分化潜能。
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| 关 键 词： | 诱导多能干细胞 类胚体 生精细胞 基因表达 |
Spontaneous differentiation potency of induced pluripotent stem cells into male germ cells in vitro |
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| LI Peng,HU Hong-liang,ZHANG Ling-ling,DING Hui,ZHANG Wei,TIAN Ru-hui,LIU Ping,SHI Zhan-ping,GUO Xi-zhi,LI Zheng,HUANG Yi-ran.Spontaneous differentiation potency of induced pluripotent stem cells into male germ cells in vitro[J].National Journal of Andrology,2011,17(1):8-12. |
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| Authors: | LI Peng HU Hong-liang ZHANG Ling-ling DING Hui ZHANG Wei TIAN Ru-hui LIU Ping SHI Zhan-ping GUO Xi-zhi LI Zheng HUANG Yi-ran |
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| Affiliation: | LI Peng1,HU Hong-liang1,ZHANG Ling-ling2,DING Hui1,ZHANG Wei1,TIAN Ru-hui1,LIU Ping1,SHI Zhan-ping1,GUO Xi-zhi2,LI Zheng1,HUANG Yi-ran11.Department of Urology,Shanghai Institute of Andrology,Shanghai Human Sperm Bank,Laboratory of Sperm Development and Genetics,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200001,China,2.BIO-X Center,Shanghai Jiaotong University,Shanghai 200240 |
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| Abstract: | Objective:To explore the expression profile of male germ cell-associated genes during the spontaneous differentiation of induced pluripotent stem cells(iPS) and assess the potency of their spontaneous differentiation into male germ cells in vitro.Methods:Embryoid body(EB) formation was used to promote the spontaneous differentiation of iPS into male germ cells,and the expressions of germ cell-associated genes were detected by real-time PCR and PCR.Results:Real-time PCR and PCR revealed different expression ... |
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| Keywords: | induced pluripotent stem cell embryoid body male germ cell gene expression |
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