红叶石楠生根培养与根系活力的研究

 以红叶石楠继代组培苗为试材, 研究了不同生长素( IAA、NAA和IBA) 、间苯三酚、暗培养、多效唑(MET) 以及培养基支持体对红叶石楠生根过程的影响。结果表明: NAA 015 mg·L ^{- 1}和暗培养7 d促进红叶石楠的生根; 培养基1/2MS +NAA 0.5 mg·L ^{- 1} + IBA 0.3 mg·L ^{- 1} +MET 0.5 mg·L ^{- 1}的处理使平均每株生根数达615条, 生根率达到90%; 以琼脂、蛭石、珍珠岩为培养基支持体诱导生根, 其中以珍珠岩为培养基支持体诱导效果最佳, 平均每株生根数达2318条, 生根率达96.6% , 移栽成活率达95.9% , 根系活力高达576.159μg·g^{- 1} ·h^{- 1} , 过氧化物酶( POD) 活性达到27.08 △A₄₇₀ ·min^{- 1} ·g^{- 1}。

The Study of Root Induction and Root Vigor in Photinia fraseri in Vitro

The effects of different auxin ( IAA, NAA and IBA) , phloroglucinol, culture in dark, paclobutrazol (MET) and supportingmaterials on Photinia fraseri rooting were studied. The results showed that NAA 0.5 mg·L ^{- 1} and culture in dark for 7 days promoted rooting through orthogonal experiment; and when the 1 /2MS +NAA 0.5 mg·L ^{- 1} + IBA 0.3 mg·L ^{- 1} +MET 0.5 mg·L ^{- 1} medium induced rooting, mean number of roots was 6.5, rooting rate was 90%. Besides, the effects of agar, veimiculite and pearlite as supporting materials were studied. Pearlite was the best supporting material; mean number of roots was 23.8; rooting rate was 9616%; the survive rate of transplant seedlings reached 95.9%; root vigor reached 576.159μg·g^{- 1} ·h^{- 1} ; POD activity reached 27.08 △A₄₇₀ ·min^{- 1} ·g^{- 1}.