PCR同时检测变形链球菌和远缘链球菌

目的:建立一种快速、简便地同时检测变形链球菌和远缘链球菌的方法。方法:以变形链球菌Dextranase基因设计引物,用PCR检测变形链球菌群细菌。结果:变形链球菌(血清型c、e、f)的PCR扩增产物为720 bp;远缘链球菌(血清型d、g)的PCR扩增产物为460 bp;道勒链球菌(血清型h)910 bp;仓鼠链球菌(血清型a),鼠链球菌(血清型b)均为1 270 bp。其它异种菌均不能扩增出产物,因此该PCR检测具有高度的特异性。从细菌纯培养物中PCR检测的敏感性为105菌落形成单位(colony-form ing un its,CFU)。结论:PCR能同时检测变形链球菌和远缘链球菌。该检测方法具有较高的敏感性和特异性,有望运用于临床检测。

Simultanous Detection of Streptococcus Mutans and Streptococcus Sobrinus by Polymerase Chain Reaction

Objective: To establish a simple and rapid method to detect Streptococcus mutans and Streptococcus sobrinus simultaneously.Methods: Chromosomal DNA from the bacteria was obtained by extracted with phenol-chloroform.PCR method with oligonucleotide primers specific for portions of Dextranase genes of S.mutans was optimized to detect S.mutans and S.sobrinus.Results: After PCR,S.mutans and S.sobrinus were specifically identified.PCR was capable of amplifying DNA fragments specific to these species from chromosomal DNA extracted from 10~5 colony-forming units cells.Conclusion: PCR could detect S.mutans and S.sobrinus rapidly and simutaneously.