| 木犀草素对LPS诱导的RAW264.7细胞COX-2及mPGES-1表达的影响 |
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| 引用本文： | 王旭光,陈根殷,陈妙萍.木犀草素对LPS诱导的RAW264.7细胞COX-2及mPGES-1表达的影响[J].中药材,2007,30(10):1263-1266. |
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| 作者姓名： | 王旭光 陈根殷 陈妙萍 |
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| 作者单位： | 广东医学院附属医院,广东湛江,524100 |
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| 基金项目： | 广东省湛江市科技攻关项目;广东医学院校科研和教改项目 |
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| 摘 要： | 目的:探讨木犀草素对脂多糖(LPS)诱导RAW264.7细胞COX-2及mPGES-1表达的影响。方法:酶免疫测定法(EIA)检测木犀草素对PGE2生成的影响,逆转录聚合酶链反应(RT-PCR)检测COX-2及mPGES-1 mRNA的表达。免疫印迹法(Western blotting)检测COX-2及mPGES-1蛋白的表达。结果:木犀草素抑制LPS诱导的RAW264.7细胞PGE2的生成,同时下调LPS诱导的RAW264.7细胞COX-2及mPGES-1 mRNA和蛋白的表达。结论:木犀草素可以抑制PGE合成途径中两个诱导酶COX-2和mPGES-1的表达。
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| 关 键 词： | 木犀草素 前列腺素E2 环氧合酶-2 膜相关前列腺素E2合成酶 |
| 文章编号： | 1001-4454（2007）10-1263-04 |
| 修稿时间： | 2007-03-29 |
Effect of Luteolin on COX-2 and mPGES-1 Expression in LPS-induced RAW264.7 Cells |
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| WANG Xu-guang,CHEN Gen-yin,CHEN Miao-ping.Effect of Luteolin on COX-2 and mPGES-1 Expression in LPS-induced RAW264.7 Cells[J].Jorunal of Chinese Medicinal Materials,2007,30(10):1263-1266. |
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| Authors: | WANG Xu-guang CHEN Gen-yin CHEN Miao-ping |
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| Affiliation: | First Affiliated Hospital of Guangdong Medical College, Zhanjiang 524100, China |
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| Abstract: | OBJECTIVE: To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells. METHODS: After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1 microg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay (EIA). The mRNA expression of COX-2 and mPGES-1 in RAW264.7 cells were analysed by RT-PCR. The COX-2 and mPGES-1 protein expression in RAW264. 7 cells were analysed by western blotting. RESULTS: Luteolin inhibited the LPS-induced PGE, synthesis in RAW264.7 cells. The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin. CONCLUSION: Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway. |
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| Keywords: | Luteolin Prostaglandin E2 Cycloxygenase-2 Membrane-associated PGE2 Synthase |
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