四株人白血病多药耐药细胞系的建立及其生物学特性的初步研究 |
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引用本文: | 石淑文,郭淑芬. 四株人白血病多药耐药细胞系的建立及其生物学特性的初步研究[J]. 中国小儿血液与肿瘤杂志, 1997, 0(5) |
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作者姓名: | 石淑文 郭淑芬 |
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作者单位: | 浙江医科大学附属儿童医院!310003 |
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摘 要: | 我们对人红白血病细胞株K562和人早幼粒细胞白血病细胞株HL-60应用高三尖杉酯破(HHT)或长春新碱(VCR),采用反复短期暴露法,并逐渐增加HHT和VCR的浓度,成功地建立了K562/VCR、K562/HHT、HL-60/VCR和HL-60/HHT四株具有高度耐药性的多药耐药(MDR)细胞系。免疫组织化学染色显示,K562/VCR、K562/HHT及HL60/VCR三株MDR细胞系有卜糖蛋白(Pgp)的高度表达,而HL-60/HHT细胞系没有pgP的表达。四株MDR细胞系均未见有多药耐药相关蛋白(MRP)的表达。5μg/ml异搏定和50μg/ml红霉素都能部分逆转K562/VCR、K562/HHT和HL-60/VCR三株MDR细胞系的耐药性,但其对HL-60/HHT细胞系的耐药性几乎无逆转作用。四株MDR细胞系与其相应敏感细胞系相比,前者对DNR的摄取均减少,外排均增加。2.5~10μg/ml异搏定能增加K562/VCR、K562/HHT和HL-60/VCR三株MDR细胞系对DNR的摄取量和滞留量,且其作用随异搏定浓度的增加而增强,但50~200μgn/ml红霉素不能增加它们对DNR的摄取量和滞留量。上述浓度异搏定或红霉素均不能增加HL-60/HHT细胞系对DNR的摄取量和滞留量。
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关 键 词: | 白血病 药物耐受性 细胞系 异搏定 红霉素 |
A PRELIMINARY STUDY ON ESTABLISHMENT AND CHARACTERIZATION OF FOUR HUMAN MULTIDRUGRESISTANT LEUKEMIC CELL LINES |
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Abstract: | We established four human highly multidrug- resistant (MDR ) leukemic cell lines (K562/VCR, K562/HHT, HK-60/VCR and HL-60/HHT) from human erythroleukemiacell line K562 and human promyelocytic leukemia cell line HL-60 by short and exposures to HHT or VCR with gradually increasing concentration of HHT or VDR. Immunohistochemical staining demonstrated K562/VCR, K562/HHT and HL-60/VCR MDR cell lines overexpressed p-glycoprotein (Pgp), but HL-60/HHT cell line didn' t. No expression of MDR-related protein (MRP) was found in all four MDR cell lines. The resistance of K562/VCR or K562/HHT or HL-60/VCR MDR cell line could be partly reversed, hut that of HL-60/HHT MDR cell line could be hardly done by 5μg/ml verapamil or 50μg/ml erythromycin.Both DNR accumulation reduced and DNR efflux increased in four MDR cell lines as compared with their parental cell lines. 2. 5~10μg/ml verapamil was able to increase DNR accumulation and retention in K562/VCR, K562/HHT and HL-60/VCR MDR cell lines, and the more verapamil, the more DNR accumulation and retention, but not in HL-60/HHT MDR cell line. 50-200μg/ml erythromycin was unable to increase NDR accumulation and retention on four MDR cell lines. |
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