Phenol degradation by a psychrotrophic strain of Pseudomonas putida |
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Authors: | Gopaul Kotturi Campbell W. Robinson William E. Inniss |
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Affiliation: | (1) Department of Chemical Engineering, University of Waterloo, Waterloo, N2L 3G1 Ontario, Canada;(2) Department of Biology, University of Waterloo, Waterloo, N2L 3G1 Ontario, Canada |
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Abstract: | Summary Cell growth and phenol degradation kinetics were studied at 10°C for a psychrotrophic bacterium, Pseudomonas putida Q5. The batch studies were conducted for initial phenol concentrations, So, ranging from 14 to 1000 mg/1. The experimental data for 14<=So<=200 mg/1 were fitted by non-linear regression to the integrated Haldane substrate inhibition growth rate model. The values of the kinetic parameters were found to be: m=0.119 h–1, KS=5.27 mg/1 and KI=377 mg/1. The yield factor of dry biomass from substrate consumed was Y=0.55. Compared to mesophilic pseudomonads previously studied, the psychrotrophic strain grows on and degrades phenol at rates that are ca. 65–80% lower. However, use of the psychrotrophic microorganism may still be economically advantageous for waste-water treatment processes installed in cold climatic regions, and in cases where influent waste-water temperatures exhibit seasonal variation in the range 10–30°C.NomenclatureKS saturation constant (mg/l) - KI substrate inhibition constant (mg/l) - specific growth rate (h–1) - m maximum specific growth rate without substrate inhibition (h–1) - max maximum achievable specific growth rate with substrate inhibition (h–1) - S substrate (phenol) concentration (mg/l) - So initial substrate concentration (mg/l) - Smax substrate concentration corresponding to max (mg/l) - t time (h) - X cell concentration, dry basis (mg DW/l) - Xf final cell concentration, dry basis (mg DW/l) - Xo initial cell concentration, dry basis (mg DW/l) - Y yield factor (mg DW cell produced/mg substrate consumed) |
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