人乳头瘤病毒线性平行杂交分型检测条件的建立及优化

目的研究人乳头瘤病毒（HPV）线性平行杂交分型检测（LiPA）条件的建立及优化。方法采用PCR方法和反向杂交相结合的技术。使扩增产物与水平固定在长条形尼龙膜上的型特异性寡核苷酸探针杂交，再利用显色系统形成肉眼可见的产物，根据产物和条膜底色的清晰度对LiPA杂交的重要参数进行优化，包括点膜的探针浓度，杂交温度等。结果建立并优化了LiPA杂交的条件，本底清晰，结果可靠。结论建立的LiPA杂交技术经济简便。切实可行。

Establishment and optimization of line probe assay(LiPA)for identification of human papillomavirus

Objective To establish and optimize the LiPA technique. Methods The LiPA technique based on the principle of reverse hybridization and the polymerase chain reaction （PCR） was established. Part of the L1 region of the human papiUomavirus （HPV）genome was amplified, and the resulting biotinylated amplicons were then denatured and hybridized with specific ohgonueleotide probes. These probes were immobilized as parallel lines on membrane strips. The visual interpretation of the signal pattern was used to optimize experiments for the LiPA including hybridization probe concentrations, hybridization temperatures et al. Results The established and optimized LiPA technique is reliable with low background. Conclusion The established LiPA technique is practicable and economical.