灵芝孢子粉破壁工艺优化及其抗肿瘤作用

以灵芝孢子粉为原料,采用高压均质法破壁灵芝孢子,研究灵芝孢子粉破壁优化工艺及破壁孢子粉抗肿瘤作用。选取水为破壁溶剂,以均质压力、均质次数、料液比为试验因素,以灵芝孢子破壁率为试验指标,通过单因素试验及正交试验对灵芝孢子破壁工艺进行优化。通过建立S180荷瘤小鼠模型,对破壁灵芝孢子粉的抗肿瘤作用进行研究。结果表明,最佳破壁工艺条件为均质压力150 MPa、均质次数3、料液比1∶100(g/m L),破壁率为94.35%。抗肿瘤实验表明,破壁灵芝孢子对小鼠S180肉瘤的抑制率为43.37%~57.59%,且肝体比和肺体比变化稳定,并可显著提高小鼠的胸腺指数和脾指数。因此,破壁灵芝孢子粉具有良好的抗肿瘤作用。

Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores

The purpose of this study was to investigate the optimum conditions for breaking Ganoderma lucidum spores
by high-pressure homogenization using water as the solvent and to evaluate the antitumor effect of the broken spores.
The optimization of three process variables including solid-to-liquid ratio, homogenization pressure, and the number
of homogenization cycles for improved percentage of broken spores was conducted through one-factor-at-a-time and
orthogonal array experiments. S180 tumor-bearing mice models were established for evaluation of the antitumor effect of
the broken G. lucidum spores. The results showed that the optimal conditions for breaking G. lucidum spores were obtained
as follows: homogenization pressure, 150 MPa; homogenization cycles, 3 times; and solid-to-liquid ratio, 1:100 (g/mL).
Under these conditions, the percentage of broken spores could be up to 94.35%. Antitumor tests showed that the percentage
inhibition of tumor growth in S180 tumor-bearing mice by the broken spores was 43.37%–57.59%, and the liver index and
lung index were little affected while the thymus index and spleen index were significantly enhanced. Therefore, broken G.
lucidum spores possessed good antitumor effect.