富硒豆豉中安赛蜜、苯甲酸、山梨酸、糖精钠、 脱氢乙酸的测定

目的建立高效液相色谱法同时测定富硒豆豉中安赛蜜、苯甲酸、山梨酸、糖精钠和脱氢乙酸的分析方法。方法样品经过沉淀剂沉淀蛋白后过滤,使用C_(18)反向色谱柱(pH1.5～9.0)分离,用甲醇-乙酸铵溶液作为流动相进行洗脱,经高效液相色谱紫外检测器和二极管阵列检测器检测,检测波长为230 nm。结果 5种添加剂具有良好的线性关系,相关系数均大于0.9990,检出限为1.0～2.0mg/kg。应用该方法检测富硒豆豉的加标回收率为90.2%～101.1%,相对标准偏差小于2.0%。结论本研究建立的方法有效可行,结果可靠,适用于富硒豆豉中5种添加剂的检测。

Determination of acesulfame potassium, benzoic acid, sorbic acid, saccharin sodium dihydrate and dehydroacetic acid in Se-enriched and fermented soyabeans

Objective To establish a method for determination of acesulfame potassium, benzoic acid, sorbic acid, saccharin sodium dihydrate and dehydroacetic acid in Se-enriched and fermented soyabeans by high performance liquid chromatography (HPLC). Methods The sample was deproteinated by precipitator and filtered, then separated on C18 column (pH 1.5-9.0) with methanol and ammonium acetate as mobile phase, then detected by HPLC with UV detector and diode array detector, and the detection wavelength was 230 nm. Results The 5 additives had a good linear relationship, and the correlation coefficients were all greater than 0.9990. The limits of detection were 1.0-2.0 mg/kg. The recoveries of Se-enriched and fermented soybeans were 90.2%-101.1%, with the relative standard deviations of less than 2.0%. Conclusion The established method is feasible and effective, and has the reliable results, which is suitable for the determination of additives in Se-enriched and fermented soyabeans.