虎杖提取物指纹图谱与其体外抗氧化作用的灰关联度分析

目的： 探索虎杖总提取物及不同极性部位指纹图谱各特征峰所代表的化学成分对其体外抗氧化作用贡献大小。 方法： 采用DPPH测定虎杖总提取物及不同极性部位的体外抗氧化活性；采用HPLC法测定其特征指纹图谱；采用灰关联度分析法研究其谱效关系。 结果： 虎杖体外抗氧化活性是其内部多种成分共同作用的结果，各成分对抗氧化作用贡献大小顺序（按特征峰编号）为8>1>12（蒽苷B）>4（虎杖苷）>2>6（白藜芦醇）>3>7>5>10>9>13>14（大黄素）>11>15（大黄素甲醚）。结合文献报道，初步确定在本实验参数条件下，关联度值低于0.6的11，14，15号色谱峰所代表的化学成分无贡献作用。 结论： 本试验对虎杖总提取物及不同极性部位抗氧化活性的谱效关系进行了初步探索，为中药药效物质基础和谱效关系研究提供了一定参考。

Grey Relational Analysis on Fingerprints of Extracts from Polygoni Cuspidati Rhizoma et Radix and Antioxidant Capacity in vitro

Objective: To investigate the contributions of components represented by characteristic peaks of fingerprints of total extract from Polygoni Cuspidati Rhizoma et Radix and its fractions of different polarity to antioxidant capacity in vitro. Method: The antioxidant capacity of total extract from Polygoni Cuspidati Rhizoma et Radix and its fractions of different polarity was evaluated using DPPH assay. HPLC method was introduced to determine fingerprints of the extracts. The correlation between fingerprint and antioxidant capacity was investigated by grey relational analysis. Result: The antioxidant capacity was resulted from the combined effect of multiple components. The order of contributions was(number of peaks) 8 >1>12(anthraglycoside B)>4(polydatin)>2>6 (resveratrol)>3>7>5>10>9>13>14(emodin)>11>15(physcion). Combined with the results in literature, peaks of No.11, 14 and 15, the grey relational values of which were lower than 0.6, made no contribution under this experimental parameter condition. Conclusion: The spectrum-effect relationship of antioxidant capacity in vitro of Polygoni Cuspidati Rhizoma et Radix was investigated preliminarily, which would provide some reference for the study of pharmacodynamic material basis and spectrum-effect relationship of traditional Chinese medicine.