| 水稻内生联合固氮细菌阴沟肠杆菌营养及其配方的研究 |
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| 引用本文： | 陈夕军,何平平,童蕴慧,纪兆林,徐敬友.水稻内生联合固氮细菌阴沟肠杆菌营养及其配方的研究[J].扬州大学学报(农业与生命科学版),2009,30(3). |
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| 作者姓名： | 陈夕军 何平平 童蕴慧 纪兆林 徐敬友 |
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| 作者单位： | 扬州大学,园艺与植物保护学院,江苏,扬州,225009 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 阴沟肠杆菌(Enterobacter cloacae)J115和G161是2株具高固氮活性的水稻内生细菌.经研究发现,2株固氮细菌的营养要求基本相似,具体配方为:2%葡萄糖或蔗糖、1%蛋白胨或2%牛肉浸膏、0.02%K_2HPO_4、0.1%酵母膏.另外,添加硝态氮(KNO_3)对2株固氮细菌的生长有明显的抑制作用,但氨态氮(NH_4)_2SO_4]则对细菌生长无影响.
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| 关 键 词： | 水稻 内生细菌 联合固氮细菌 阴沟肠杆菌 营养 |
Nutrient requirement and media of associative nitrogen-fixing bacterium Enterobncter cloace in rice plants |
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| CHEN Xi-jun,HE Ping-ping,TONG Yun-hui,JI Zhao-lin,XU Jing-you.Nutrient requirement and media of associative nitrogen-fixing bacterium Enterobncter cloace in rice plants[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2009,30(3). |
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| Authors: | CHEN Xi-jun HE Ping-ping TONG Yun-hui JI Zhao-lin XU Jing-you |
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| Abstract: | Isolates-J115 and G161 of Enterobacter cloace(Jordan)Hormaeche and Edwards were two rice endophytic bacteria with associative nitrogen fixation.There was only little difference between the two isolates nutrient requirement.The best formulation of the media was studied and determined,which was composed of 2% glucose or sucrose,1% peptone or 2% beef extract,0.02% K_2HPO_4 and 0.1% yeast extract.Furthermore,KNO_3 had obvious inhibition to the bacterial growth,but(NH_4)_2SO_4 had no influence. |
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| Keywords: | rice endophytic bacteria associative nitrogen-fixing bacteria Enterobacter cloace nutrition |
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