光谱法研究埃洛石纳米管与溶菌酶间的相互作用

以溶菌酶作为模型蛋白,主要利用光谱法研究了埃洛石纳米管与溶菌酶之间的相互作用。荧光光谱结果表明向溶菌酶体系中加入埃洛石纳米管会出现荧光猝灭现象,猝灭机理符合静态猝灭规律。共振光散射强度的增加可能与埃洛石纳米管-溶菌酶复合物的形成而导致的分子尺寸的增加有关,这与紫外-可见吸收光谱的变化和静态猝灭机理相一致。同步荧光光谱分析表明两者之间的相互作用可能发生在色氨酸所处位置附近,作用过程使溶菌酶的二级结构发生变化,分子链错误折叠,加入浓度为100mg/L和200mg/L的埃洛石纳米管时,通过圆二色谱数据计算出分别导致α-螺旋的含量降低3.28%和6.89%。

Spectroscopic Study on the Interaction between Halloysite Nanotubes with Lysozyme

In this article,the interaction between halloysite nanotube and lysozyme used as model protein was studied by spectroscopic method.The fluorescence of lysozyme was quenched upon addition of halloysite nanotubes and the quenching mechanism belonged to static quenching.Intensity increase of resonance light scattering may be caused by the increase of molecular size as a result of halloysite nanotube-lysozyme complex formation,which was consistent with the data of UV-vis absorption spectra and static quenching mechanism.Interaction site between halloysite nanotubes and lysozyme was around the tryptophan in lysozyme based on synchronous fluorescence spectra,where the secondary structure of lysozyme was changed.When the concentrations of halloysite nanotubes were 100 mg/mL and 200 mg/mL,the decreased a-helix structure content were 3.28％ and 6.89％,respectively,calculated by the data of circular dichroism spectra.