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| 肾型鸡传染性支气管炎病毒X株S1基因序列的测定及同源性分析 | |
| 引用本文： | 赵宝华,李尚波,王文成,尹广东,卫广森,许崇波. 肾型鸡传染性支气管炎病毒X株S1基因序列的测定及同源性分析[J]. 中国兽医学报, 2002, 22(5): 445-447 |
| 作者姓名： | 赵宝华 李尚波 王文成 尹广东 卫广森 许崇波 |
| 作者单位： | 1. 河北师范大学生命科学学院,河北石家庄,050016 2. 辽宁省益康生物制品厂,辽宁辽阳,111000 3. 宁夏大学生物工程系,宁夏银川,750021 |
| 基金项目： | 河北师范大学博士后基金资助项目 ( 2 0 0 1B0 3) |
| 摘 要： | 根据国外已发表的鸡传染性支气管炎病毒 (IBV) S1基因序列 ,设计了 2对引物并以 RT- PCR特异性扩增出IBV X株的 S1基因 ,基因产物大小为 1.6 4kb,与设计相符 ,对其进行序列测定后 ,与标准毒株 H5 2、H12 0、M41、BEAU和澳大利亚 T株的 S1基因进行同源性比较。结果表明 ,X株与 H5 2、H12 0、M41、BEAU和澳大利亚 T株的核苷酸序列的同源性分别为 75 .8%、76 .1%、76 .3%、75 .5 %和 76 .9%,由此可以看出 ,IBV X株与标准毒株在 S1基因上存在较大差异。 |
| 关 键 词： | IBV S1基因 核苷酸序列 同源性 |
| 文章编号： | 1005-4545(2002)05-0445-03 |
| 修稿时间： | 2002-02-10 |
Sequencing and Homology Analysis of the S1 Gene of Nephropathogenic Strain X |
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| Abstract: | Two pair of primers for amplifying the S1 gene of avian infectious bronchitis virus (IBV)had been designed,with which the predicted 1.62 kb fragment had been amplified by RT-PCR and RT-NPCR from Nephropathogenic Strain X.The S1 gene of Nephropathogenic Strain X was sequenced and compared with standard strains such as H52,M41,H120,BEAU and T.The results showed that the homologies of nucleic acids between X strain and H52,M41,H120,BEAU,T strains were 76.1%,76.3%,75.8%, 75.5% and 76.9% respectively.The difference of the S1 gene of Nephropathogenic Strain X with those of other IBV standard strains had been proved. |
| Keywords: | IBV S1 gene nucleotide sequence homology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
