High-performance liquid chromatographic separation of ascorbic acid,erythorbic acid,dehydroascorbic acid,dehydroerythorbic acid,diketogulonic acid,and diketogluconic acid |
| |
Authors: | Landis W Doner Kevin B Hicks |
| |
Affiliation: | Eastern Regional Research Center, Agricultural Research, Science and Education Administration, U. S. Department of Agriculture, Philadelphia, Pennsylvania 19118 USA |
| |
Abstract: | High-performance liquid chromatography on a Zorbax NH2 analytical column, with acetonitrile: 0.05 m KH2PO4 (75:25, ) used as eluant, has allowed the separation, in less than 14 min, of ascorbic acid, erythorbic acid, dehydroascorbic acid, dehydroerythorbic acid, diketogulonic acid, and diketogluconic acid. Ultraviolet monitoring at 268 nm allows ascorbic acid and erythorbic acid to be detected at the 25-ng level, while refractive index detection monitors the elution of all six compounds. Tyrosine is a good internal standard, being well separated from the other compounds and having an adequate ultraviolet absorption at 268 nm. We have found dithiothreitol to be effective in rapidly reducing dehydroascorbic acid to ascorbic acid, providing the basis for indirectly determining dehydroascorbic acid after its reduction. The potential of this high-performance liquid chromatographic procedure for evaluating the levels of these compounds in orange juice and urine is demonstrated. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|